Phylogenetic analysis of the Wnt gene family:: Insights from lophotrochozoan members
Phylogenetic analysis of the Wnt gene family:: Insights from lophotrochozoan members
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DOI：
10.1016/s0960-9822(02)01068-0
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发表时间：
2002-08-20
期刊：
影响因子：
9.2
通讯作者：
Vervoort, M
中科院分区：
文献类型：
--
作者：
Prud'homme, B;Lartillot, N;Vervoort, M
Wnt基因家族编码分泌的信号分子，其在动物发育期间控制细胞命运特化、增殖、极性和运动[1-3]。我们在这里调查这个大的多基因家族的进化历史。Wnt基因几乎完全分离自两侧动物的三个主要亚类中的两个[4]，后口动物（包括脊索动物和棘皮动物）和蜕皮动物（例如，节肢动物和线虫）。然而，后口动物和蜕皮动物Wnt基因之间的直系关系，以及更普遍的Wnt家族的同源性，尚不清楚。我们在这里报告了从两个物种（环节动物Platynereis dumerilii和软体动物Patella vulgata）中分离到的几个Wnt基因，这两个物种都属于第三个大型双侧分支，即脊轮毛动物（与蜕皮动物一起构成原口动物）。这些序列与大量其他Wnt基因序列的多重系统发育分析，特别是人、线虫和蝇的完整Wnt基因组[2]，使我们能够将Wnt家族细分为12个亚家族。其中至少有9个已经存在于所有两侧对称动物的最后一个共同祖先中，这进一步突出了这个祖先的遗传复杂性。我们在这里提出的直系关系为未来的发展比较打开了新的视角。
The Wnt gene family encodes secreted signaling molecules that control cell fate specification, proliferation, polarity, and movements during animal development [1-3]. We investigate here the evolutionary history of this large multigenic family. Wnt genes have been almost exclusively isolated from two of the three main subdivisions of bilaterian animals [4], the deuterostomes (which include chordates and echinoderms) and the ecdysozoans (e.g., arthropods and nematodes). However, orthology relationships between deuterostome and ecdysozoan Wnt genes, and, more generally, the phylogeny of the Wnt family, are not yet clear. We report here the isolation of several Wnt genes from two species, the annelid Platynereis dumerilii and the mollusc Patella vulgata, which both belong to the third large bilaterian clade, the lophotrochozoans (which constitute, together with ecdysozoans, the protostomes). Multiple phylogenetic analyses of these sequences with a large set of other Wnt gene sequences, in particular, the complete set of Wnt genes of human, nematode, and fly [2], allow us to subdivide the Wnt family into 12 subfamilies. At least nine of them were already present in the last common ancestor of all bilaterian animals, and this further highlights the genetic complexity of this ancestor. The orthology relationships we present here open new perspectives for future developmental comparisons.