HOMONUCLEAR H-1 DOUBLE-RESONANCE DIFFERENCE SPECTROSCOPY OF THE RAT-BRAIN INVIVO
HOMONUCLEAR H-1 DOUBLE-RESONANCE DIFFERENCE SPECTROSCOPY OF THE RAT-BRAIN INVIVO
复制标题
DOI：
10.1073/pnas.81.20.6330
复制
发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
SHULMAN, RG
中科院分区：
文献类型：
--
作者：
ROTHMAN, DL;BEHAR, KL;SHULMAN, RG
360.13 MHz 的 1H 同核双共振差异光谱用于解析完整大鼠大脑中的特定代谢物共振。解决的代谢物共振包括以前模糊的 Ala、GABA、Glu 和 Tau 质子共振。在活体大鼠中观察到差异光谱中的GABAα-和γ-CH2质子共振在活体大鼠中观察到在1.91ppm的β-CH2质子共振照射时获得的差异光谱。死亡后30分钟观察到GABA的α-和γ-CH2共振强度增加了3倍。 Glu的α-CH和γ-CH2共振也可在体内通过选择性照射它们自旋耦合的β-CH2质子来解析。该技术用于通过大鼠的完整头皮观察乳酸的β-CH3质子。头皮组织产生的大脂质信号在差异光谱中被消除，揭示了乳酸β-CH3共振。
1H homonuclear double-resonance difference spectroscopy at 360.13 MHz was used to resolve specific metabolite resonances in the brains of intact rats. Metabolite resonances resolved include previously obscured proton resonances of Ala, GABA, Glu and Tau. The GABA .alpha.- and .gamma.-CH2 proton resonances were observed in the living rat in the difference spectrum were observed in the living rat in the difference spectrum obtained upon irradiation of the .beta.-CH2 proton resonance at 1.91 ppm. A 3-fold increase in the intensity of the .alpha.- and .gamma.-CH2 resonances of GABA was observed 30 min after death. The .alpha.-CH and .gamma.-CH2 resonances of Glu were also resolved in vivo by selective irradiation of the .beta.-CH2 protons to which they are spin-coupled. This technique was used to observe the .beta.-CH3 protons of lactate through the intact scalp of a rat. Large lipid signals arising from scalp tissue were eliminated in the difference spectrum, revealing the lactate .beta.-CH3 resonance.