A Role for Xvax2 in Controlling Proliferation of Xenopus Ventral Eye and Brain Progenitors
A Role for Xvax2 in Controlling Proliferation of Xenopus Ventral Eye and Brain Progenitors
复制标题
Xvax2 在控制爪蟾腹眼和脑祖细胞增殖中的作用
DOI：
10.1002/dvdy.21763
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发表时间：
2008-11-01
影响因子：
2.5
通讯作者：
He, Rongqiao
中科院分区：
文献类型：
--
作者：
Liu, Mingnan;Liu, Yang;He, Rongqiao
Vax 2同源盒基因在眼睛的早期背腹图案中起着至关重要的作用。然而，尽管Vax 2转录本在后来分化的眼睛和大脑区域中被检测到，但其在这些阶段的可能作用仍不清楚。通过免疫组织化学和原位杂交，我们广泛比较了非洲爪蟾Vax 2（Xvax 2）mRNA和蛋白的表达模式。Xvax 2蛋白的表达被发现在很大程度上是重叠的，但比mRNA的表达更受限制，这表明Xvax 2的表达也可能在转录后水平受到调控。在眼和脑神经发生过程中，Xvax 2蛋白在两种结构的腹侧区域的增殖神经祖细胞和有丝分裂后分化细胞中检测到。通过mRNA显微注射和DNA脂质体转染在非洲爪蟾胚胎中过表达Xvax 2似乎抑制眼和脑细胞的增殖，从而指出Vax 2在控制腹侧眼和脑祖细胞的增殖特性中的新的潜在作用。发展动力学237：3387-3393，2008年。(C)2008威利利斯公司
The Vax2 homeobox gene plays a crucial role in early dorsoventral patterning of the eye. However, although Vax2 transcripts have been detected in later differentiating eye and brain regions, its possible roles at these stages are still unclear. By immunohistochemistry and in situ hybridization, we extensively compared the expression patterns of Xenopus Vax2 (Xvax2) mRNA and protein. Expression of Xvax2 protein was found to be largely overlapping but more restricted than that of mRNA, suggesting that Xvax2 expression may be also regulated at posttranscriptional levels. During eye and brain neurogenesis, Xvax2 protein was detected in proliferating neural progenitors and postmitotic differentiating cells in ventral regions of both structures. Overexpression of Xvax2 in Xenopus embryos by mRNA microinjection and DNA lipofection appeared to inhibit proliferation in both eye and brain cells, thus pointing to a new potential role for Vax2 in controlling the proliferative properties of ventral eye and brain progenitors. Developmental Dynamics 237:3387-3393, 2008. (C) 2008 Wiley-Liss, Inc.