LIGHT-MICROSCOPE STUDY OF MIXED HELICES IN RECONSTITUTED SALMONELLA FLAGELLA
LIGHT-MICROSCOPE STUDY OF MIXED HELICES IN RECONSTITUTED SALMONELLA FLAGELLA
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DOI：
10.1016/0022-2836(76)90305-3
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发表时间：
1976-01-01
影响因子：
5.6
通讯作者：
HOTANI, H
中科院分区：
文献类型：
--
作者：
HOTANI, H
用暗视野显微镜拍摄溶液中的单个鞭毛细丝，以确定描述其完整形状的参数。根据对沙门氏菌SJ25和SJ814鞭毛蛋白共聚物所假定的螺旋I、II和III螺距的测量，结合每根螺旋的每周期轮廓长度的EM数据，计算了这3个螺旋的螺距。(俯仰角是灯丝的切线和螺旋轴之间的角度。)以SJ25和SJ814鞭毛蛋白为原料，采用两步共聚法制备了由两个不同螺旋结构的嵌段组成的纤维，并对其形态进行了分析。在任何混合类型的灯丝中，组成块的轴线以相同的角度定向，称为块角度.PSI。该角度近似为PSI=180度。-.**图形**。其中，α1和β2是混合螺旋的螺距角。在此基础上，讨论了混合型细丝的形态。
Single flagellar filaments in solutions were photographed by dark-field microscopy to determine parameters to describe their intact shapes. From the measurement of pitches of helices I, II and III assumed by copolymers of flagellins from Salmonella strains SJ25 and SJ814 combined with EM data of the contour length per period of each filament, the pitch angles of the 3 helices were calculated. (The pitch angle is the angle between a tangent to the filament and the helical axis.) The filaments which consisted of 2 blocks assuming different helices were prepared by 2-step copolymerization of SJ25 and SJ814 flagellins and the configurations of these mixed-type filaments were examined. In filaments of any mixed type, the axes of the constituent blocks were oriented at the same angle, called the block angle .PSI.. This angle was approximated by .PSI. = 180.degree. - .**GRAPHIC**. where .theta.1 and .theta.2 are the pitch angles of the mixed helices. On the basis of this finding, the morphology of mixed-type filaments is discussed.