Dopaminergic stimulation of cAMP accumulation in cultured rat mesangial cells.
Dopaminergic stimulation of cAMP accumulation in cultured rat mesangial cells.
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多巴胺能刺激培养的大鼠系膜细胞中 cAMP 的积累。
DOI：
10.1152/ajpheart.1987.253.2.h358
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Abboud,HE
中科院分区：
文献类型：
--
作者：
Shultz,PJ;Sedor,JR;Abboud,HE
多巴胺（DA）改变肾脏血流动力学，DA受体已在离体肾小球中被证实。为了确定携带DA受体的肾小球细胞类型，我们研究了多巴胺能激动剂和拮抗剂对培养的大鼠肾小球系膜和上皮细胞中腺苷3′，5′-环单磷酸腺苷（cAMP）积累的影响。DA引起系膜细胞而非上皮细胞cAMP积累的明显剂量和时间依赖性增加。DA的刺激作用可被DA拮抗剂氟哌啶醇、三氟拉嗪和顺式硫代噻吩所消除，但不能被-或-肾上腺素能或组胺拮抗剂所消除。与DA的作用类似，两种多巴胺I型受体激动剂非诺多巴胺和SKF 38393显著刺激了cAMP在系膜细胞中的积累。此外，DA的作用被特异性DA1受体拮抗剂Sch 23390阻断，而不被特异性DA2拮抗剂多潘立酮阻断。这些结果表明，DA通过da1型受体调控cAMP在系膜细胞中的积累。
Dopamine (DA) alters renal hemodynamics, and DA receptors have been demonstrated in isolated glomeruli. To determine the glomerular cell type bearing DA receptors, we studied the effect of dopaminergic agonists and antagonists on adenosine 3',5'-cyclic monophosphate (cAMP) accumulation in rat glomerular mesangial and epithelial cells in culture. DA caused a marked dose- and time-dependent increase in cAMP accumulation in mesangial but not epithelial cells. The stimulatory effect of DA was abolished by the DA antagonists haloperidol, trifluoperazine, and cis-thiothixene but not by beta- or alpha-adrenergic or histamine antagonists. Similar to the effects of DA, two dopamine type I receptor agonists, fenoldopam and SKF 38393, markedly stimulated cAMP accumulation in the mesangial cells. Moreover, the effects of DA were blocked by Sch 23390, a specific DA1 receptor antagonist, but not domperidone, a specific DA2 antagonist. These results show that DA regulates cAMP accumulation in mesangial cells via DA1-type receptors.