A Comprehensive Proteomics and Transcriptomics Analysis of Bacillus subtilis Salt Stress Adaptation
A Comprehensive Proteomics and Transcriptomics Analysis of Bacillus subtilis Salt Stress Adaptation
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DOI：
10.1128/jb.01106-09
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发表时间：
2010-02-01
影响因子：
3.2
通讯作者：
Becher, Doerte
中科院分区：
文献类型：
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作者：
Hahne, Hannes;Maeder, Ulrike;Becher, Doerte
在其自然栖息地中，枯草芽孢杆菌暴露于不断变化的渗透压，需要适应性应激反应。转录组学和蛋白质组学方法可以提供盐胁迫B中发生的动态变化的图片。枯草杆菌培养物，因为这些研究提供了细胞应对高盐度的公正观点。我们应用全基因组微阵列技术和代谢标记，结合最先进的蛋白质组学技术，提供了一个全球性的和时间分辨的图片的生理反应的B。枯草杆菌细胞暴露于严重的和突然的渗透上移。这种组合的实验方法提供了3，961个mRNA转录谱、590个在胞质溶胶中检测到的蛋白质表达谱和383个在膜组分中检测到的蛋白质表达谱的定量数据。我们的研究揭示了一个良好的协调诱导的基因表达后，渗透上调，涉及大部分的SigB，SigW，SigM和SigX调节子。此外，观察到大量不属于这些调节子的基因的渗透上调。总的来说，渗透上调约500 B。检测到枯草芽孢杆菌基因。我们的数据为进一步深入研究B的生理和遗传反应提供了前所未有的丰富基础。枯草芽孢杆菌对高渗胁迫的反应。
In its natural habitats, Bacillus subtilis is exposed to changing osmolarity, necessitating adaptive stress responses. Transcriptomic and proteomic approaches can provide a picture of the dynamic changes occurring in salt-stressed B. subtilis cultures because these studies provide an unbiased view of cells coping with high salinity. We applied whole-genome microarray technology and metabolic labeling, combined with state-of-the-art proteomic techniques, to provide a global and time-resolved picture of the physiological response of B. subtilis cells exposed to a severe and sudden osmotic upshift. This combined experimental approach provided quantitative data for 3,961 mRNA transcription profiles, 590 expression profiles of proteins detected in the cytosol, and 383 expression profiles of proteins detected in the membrane fraction. Our study uncovered a well-coordinated induction of gene expression subsequent to an osmotic upshift that involves large parts of the SigB, SigW, SigM, and SigX regulons. Additionally osmotic upregulation of a large number of genes that do not belong to these regulons was observed. In total, osmotic upregulation of about 500 B. subtilis genes was detected. Our data provide an unprecedented rich basis for further in-depth investigation of the physiological and genetic responses of B. subtilis to hyperosmotic stress.