Protein and surface effects on monocyte and macrophage adhesion, maturation, and survival
Protein and surface effects on monocyte and macrophage adhesion, maturation, and survival
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DOI：
10.1002/jbm.10043
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发表时间：
2002-06-05
期刊：
影响因子：
--
通讯作者：
Anderson, JM
中科院分区：
文献类型：
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作者：
Collier, TO;Anderson, JM
细胞的黏附和成熟会受到植入生物材料表面蛋白质吸附特性的影响。在这项研究中，我们研究了表面化学和吸附蛋白如何在体外调节单核细胞和巨噬细胞的黏附、IL-13诱导的异物巨细胞形成和细胞凋亡。与二甲基硅烷修饰的表面(DM)相比，RGD多肽修饰的表面在非耗竭血清条件下对黏附密度、异体巨细胞(FBGC)的形成和细胞凋亡没有影响。特异性黏附蛋白的耗尽影响黏附、FBGC的形成和细胞凋亡。去除血清中的纤维粘连蛋白和玻璃体连结蛋白与未去除血清的情况相比并无总体影响，但去除血清中的免疫球蛋白导致糖尿病患者的初始贴壁细胞密度显著降低[1000+/-200比2460+/-590(p=0.02)]，FBGC形成显著减少[2%比17%(p=0.02)]，细胞凋亡率显著增加[57%比32%(p=0.01)]。在RGD表面没有观察到DM表面初始黏附细胞密度的降低，这表明RGD表面促进了初始黏附的增加。然而，RGD表面不影响FBGC的形成(即巨噬细胞融合)或凋亡水平，这在第7天和第10天仍然与DM表面上的水平相当。(C)2002年Wiley期刊公司。
Cell adhesion and maturation can be affected by the protein adsorption profile on the surface of an implanted biomaterial. In this study we have investigated how surface chemistry and adsorbed proteins can modulate monocyte and macrophage adhesion, IL-13-induced foreign-body giant cell formation, and apoptosis in vitro. Compared to a dimethylsilane-modified surface (DM), a surface modified with RGD peptides had no effect on adhesion density, foreign-body giant cell (FBGC) formation, or apoptosis in non-depleted serum conditions. The depletion of specific adhesive proteins affected adhesion, FBGC formation, and apoptosis. While the depletion of fibronectin and vitronectin had no overall effect compared to nondepleted serum conditions, the depletion of IgG from serum caused a significant decrease in initial adherent cell density [1000 +/- 200 compared to 2460 +/- 590 (p = 0.02)], a significant decrease in FBGC formation [2% compared to 17% (p = 0.02)], and a significant increase in the level of apoptosis [57% compared to 32% (p = 0.01)] on DM. The lowered initial adherent cell density on DM was not observed on the RGD surface, indicating that the RGD surface promotes increased initial adhesion. However, the RGD surface does not affect FBGC formation (i.e., macrophage fusion) or levels of apoptosis, which remained comparable to those on the DM surfaces at days 7 and 10. (C) 2002 Wiley Periodicals, Inc.