Communication compartments in mixed cell cultures.
Communication compartments in mixed cell cultures.
复制标题
混合细胞培养物中的通讯隔室。
DOI：
10.1016/0014-4827(85)90550-6
复制
发表时间：
1985
影响因子：
3.7
通讯作者：
E. Kam
中科院分区：
文献类型：
--
作者：
J. Pitts;E. Kam
上皮细胞（BRL）和成纤维细胞（BHK）的混合培养物，它们将自己分成每种细胞类型的不同区域，形成通讯区室。电偶联、染料偶联和代谢偶联测量已被用于表明小离子和分子可以通过域中所有细胞之间的细胞间连接自由移动，而它们跨越域之间的边界的移动受到严格限制。代谢偶联是最敏感的方法检测trans-boundary communication，但从所有三种方法得到的结果是兼容的。数据表明，跨边界的转移减少是由于通道较少，这是由于异源细胞之间连接形成的频率较低，而不是由于通道直径较小。细胞质小分子的浓度梯度可以建立在这些通信隔间，这是类似于那些预测解释发展中系统中的模式形成。有人建议，细胞表面的功能，导致这种整理也是负责异源接点形成的频率降低，因此区室化。
Mixed cultures of epithelial (BRL) cells and fibroblasts (BHK), which sort themselves out into separate domains of each cell type, form communication compartments. Electrical coupling, dye coupling and metabolic coupling measurements have been used to show that small ions and molecules can move freely via intercellular junctions between all the cells in a domain, while their movement across the boundaries between domains is severely restricted. Metabolic coupling is the most sensitive method for detectingtrans-boundary communication but the results obtained from all three methods are compatible. The data suggest the reduced transfer across the boundaries is due to fewer channels, resulting from a lower frequency of junction formation between heterologous cells, rather than to channels of smaller diameter. Concentration gradients of small cytoplasmic molecules can be established within these communication compartments which are similar to those predicted to explain pattern formation in developing systems. It is suggested that the cell surface features which cause this sorting out are also responsible for the reduced frequency of heterologous junction formation and hence for compartmentalization.