Human gastric epithelium produces IL-4 and IL-4δ2 isoform only upon Helicobacter pylori infectione
Human gastric epithelium produces IL-4 and IL-4δ2 isoform only upon Helicobacter pylori infectione
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DOI：
10.1177/039463200702000417
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发表时间：
2007-10-01
影响因子：
3.5
通讯作者：
D'Elios, M. M.
中科院分区：
文献类型：
--
作者：
Orsini, B.;Vivas, J. R.;D'Elios, M. M.
最近的证据表明，白细胞介素-4（IL-4）与粘膜耐受性有关，通过粘膜耐受性，损伤性免疫应答被预防、抑制或转变为非损伤性应答。我们研究了IL-4及其剪接变体亚型IL-4 δ 2在健康人和幽门螺杆菌感染的胃炎患者胃上皮细胞中的表达。pylori），有或没有cag致病岛（cag-PAI）。IL-4和IL-4 delta 2 mRNA在显微解剖的胃上皮和与H. pylori B128或SSI菌株。IL-4 mRNA在健康受试者的胃粘膜上皮细胞中检测到。IL-4 mRNA在H. pylori感染者，cag-PAI阳性者明显降低。IL-4 δ 2mRNA在H.幽门螺杆菌感染的患者，但不是在健康受试者。cag-PAI阳性组IL-4 δ 2表达低于cag-PAI阴性组。pylori感染的患者。AGS细胞在SS 1菌株刺激后也产生IL-4 mRNA，而在与SS 1或B128菌株共培养的AGS中检测到IL-4 δ 2 mRNA表达。胃粘膜上皮细胞IL-4和IL-4 delta 2 mRNA表达与胃炎评分呈负相关。IL-4，而不是IL-4 δ 2，在健康受试者的胃上皮中表达，而IL-4 δ 2和较少的IL-4 mRNA在H.幽门螺杆菌感染患者提示胃上皮细胞可能通过调节IL-4和IL-4 delta 2的表达来调节耐受和免疫应答之间的平衡。
Recent evidence suggests that interleukin-4 (IL-4) is related to mucosal tolerance by which an injurious immune response is prevented, suppressed or shifted to a non-injurious response. We investigated the expression of IL-4 and its splice variant isoform IL-4 delta 2 in gastric epithelial cells of healthy subjects and gastritis patients infected with Helicobacterpylori (H. pylori) with or without the cag pathogenicity island (cag-PAI). IL-4 and IL-4 delta 2 mRNAs were evaluated in microdissected gastric epithelium and in AGS cell lines co-cultured with H. pylori B128 or SSI strains. IL-4 mRNA was consistently detected in microdissected gastric epithelial cells from healthy subjects. The IL-4 mRNA expression was low in H. pylori-infected patients, and markedly reduced in cag-PAI-positive ones. IL-4 delta 2 mRNA was expressed on gastric epithelium of H. pylori-infected patients, but not in healthy subjects. The IL-4 delta 2 expression was lower in cag-PAI-positive than in cag-PAI-negative H. pylori infected patients. AGS cells also produced IL-4 mRNA upon SSI strain stimulation, whereas IL-4 delta 2 mRNA expression was detected in AGS co-cultured with either SS] or B128 strains. An inverse correlation was documented between IL-4 and IL-4 delta 2 mRNA expression by microdissected gastric epithelial cells and the score of gastritis. IL-4, but not IL-4 delta 2, is expressed by gastric epithelium of healthy subjects, whereas IL-4 delta 2 and lesser IL-4 mRNA are detectable in the gastric epithelium of H. pylori-infected patients. Data suggest that gastric epithelial cells might regulate the balance between tolerance and immune response by the fine tuning of IL-4 and IL-4 delta 2 expression.