A specific phosphoprotein phosphatase acts on histone H1 phosphorylated by protein kinase C.
A specific phosphoprotein phosphatase acts on histone H1 phosphorylated by protein kinase C.
复制标题
一种特定的磷蛋白磷酸酶作用于被蛋白激酶 C 磷酸化的组蛋白 H1。
DOI：
10.1073/pnas.80.22.6760
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发表时间：
1983
影响因子：
11.1
通讯作者：
P. Cuatrecasas
中科院分区：
文献类型：
--
作者：
N. Sahyoun;H. Levine;R. McConnell;D. Bronson;P. Cuatrecasas
一种来自大鼠肝胞质溶胶的磷酸组蛋白磷酸酶特异性作用于组蛋白H1，组蛋白H1被Ca 2 +-磷脂依赖性蛋白激酶（蛋白激酶C）磷酸化。32 P标记的H1的表观Km为1 microM;其他组蛋白或胞质蛋白与蛋白激酶C或环AMP依赖性蛋白激酶磷酸化是磷酸酶的不良底物。该酶已被部分纯化的凝胶渗透色谱法，并通过利用高效液相色谱离子交换柱。该酶的物理性质包括5.0 nm的斯托克斯半径、7.0 S的沉降系数（s20，w）和150，000的Mr。纯化的大鼠肝细胞核中的蛋白激酶以及特异性磷酸化组蛋白磷酸酶的检测表明，蛋白激酶C和相应的磷酸化组蛋白磷酸酶介导的组蛋白H1磷酸化-去磷酸化循环的生理作用。
A phosphohistone phosphatase from rat liver cytosol acts specifically on histone H1 that is phosphorylated with the Ca2+-phospholipid-dependent protein kinase (protein kinase C). The apparent Km for 32P-labeled H1 is 1 microM; other histones or cytosolic proteins phosphorylated with protein kinase C or with cyclic AMP-dependent protein kinase are poor substrates for the phosphatase. The enzyme has been partially purified by gel-permeation chromatography and by utilizing a high-performance liquid chromatography ion-exchange column. The physical properties of this enzyme include a Stokes radius of 5.0 nm, a sedimentation coefficient (s20,w) of 7.0 S, and a Mr of 150,000. The detection of protein kinase as well as the specific phosphohistone phosphatase in purified rat liver nuclei suggests a physiologic role for a histone H1 phosphorylation-dephosphorylation cycle mediated by protein kinase C and the corresponding phosphohistone phosphatase.