DNA damage production in CHO cells at elevated temperatures.
DNA damage production in CHO cells at elevated temperatures.
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CHO 细胞在高温下产生 DNA 损伤。
DOI：
10.1002/jcp.1041240318
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发表时间：
1985
影响因子：
5.6
通讯作者：
Axtell-Bartlett,J
中科院分区：
文献类型：
--
作者：
Warters,RL;Brizgys,LM;Axtell-Bartlett,J
用碱性滤膜洗脱法和碱性蔗糖梯度沉降法观察了高温对中国仓鼠卵巢细胞核DNA损伤的诱导作用。这些损伤主要在温度高于45°C时观察到，活化能为140千卡/摩尔。在碱性蔗糖梯度上，细胞基因组被还原为140 S或2 × 108道尔顿的DNA亚基，而在45°C以上的温度下，不依赖于暴露时间的增加。大的热活化能和有限的DNA大小的减少表明可能参与的热变性的核多肽在这些核病变的生产。
Induction of DNA lesions in the nucleus of Chinese hamster ovary (CHO) cells was observed at hyperthermic temperatures using the alkaline filter elution and the alkaline sucrose gradient sedimentation methods. These lesions were observed principally at temperatures greater than 45°C with an activation energy of 140 kcal/mole. On alkaline sucrose gradients the cell genome was reduced to a 140 S or 2 × 108dalton subunit of DNA independent of increasing exposure time at temperatures above 45°C. The large thermal activation energy and the limited DNA size reduction suggest the possible involvement of thermal denaturation of a nuclear polypeptide in the production of these nuclear lesions.