Isolation of a cellular subpopulation from a human colonic carcinoma cell line.
Isolation of a cellular subpopulation from a human colonic carcinoma cell line.
复制标题
从人结肠癌细胞系中分离细胞亚群。
作者：
P. Kimball;M. Brattain
从人结肠癌细胞系HT 29的培养物中分离出对5-氟尿嘧啶具有增加的抗性的细胞群。耐药细胞（HTFU）在光镜和电镜下显示出改变的形态，并在体外表现出接触抑制。DNA分析和染色体计数表明，HT 29文化表现出超和亚非整倍体，而HTFU文化似乎完全亚非整倍体。HTFU分离后一年，两种细胞系对5-氟脱氧尿苷的敏感性相同，而HTFU细胞对5-氟尿嘧啶的敏感性相对较低。癌胚抗原的生产没有表现出前或汇合后的培养物的HTFU，虽然癌胚抗原存在于细胞和媒体的HT 29文化。两种培养物均显示在半固体培养基中生长;然而，HTFU的平板接种效率低于HT 29。在注射HT 29或HTFU细胞的所有裸鼠中观察到肿瘤。由HTFU细胞形成的肿瘤较小，并且通常在6至8周后原发部位消退;尽管体外试验表明HTFU细胞的致瘤潜力降低，但仅在注射HTFU细胞系的小鼠中观察到转移。
A population of cells with increased resistance to 5-fluorouracil was isolated from cultures of the human colonic carcinoma cell line HT29. The resistant cells (HTFU) showed an altered morphology by light and electron microscopy and demonstrated contact inhibition in vitro. DNA assays and chromosome counts showed that HT29 cultures exhibit both hyper- and hypoaneuploidy, while HTFU cultures appear exclusively hypoaneuploid. One year after the isolation of HTFU, both cell lines showed equal sensitivity to 5-fluorodeoxyuridine while HTFU cells retained comparative insensitivity to 5-fluorouracil. Carcinoembryonic antigen production was not demonstrated in pre- or postconfluent cultures of HTFU, although carcinoembryonic antigen was present in both cells and media of HT29 cultures. Growth in semisolid medium was demonstrated for both cultures; however, HTFU showed a lower plating efficiency than did HT29. Tumors were observed in all of the nude mice given injections of HT29 or HTFU cells. Tumors formed from HTFU cells were smaller, and frequently the primary site receded after 6 to 8 weeks; Although in vitro tests suggested a reduced tumorigenic potential for HTFU cells, metastasis was observed only in mice given injections of cells from the HTFU line.