Isolation of a novel prolactin-like cDNA clone from developing rat placenta.
Isolation of a novel prolactin-like cDNA clone from developing rat placenta.
复制标题
从发育中的大鼠胎盘中分离出新型催乳素样 cDNA 克隆。
DOI：
10.1016/s0021-9258(18)67469-1
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
H. Friesen
中科院分区：
文献类型：
--
作者：
M. L. Duckworth;L. Peden;H. Friesen
中期至晚期大鼠胎盘以特定的时间方式产生几种中等丰度的蛋白质，其中一种我们已经鉴定为大鼠胎盘催乳素II（rPLII）（Duckworth，M. L.，Kirk，K. L.，和弗里森，H. G.（1986）J.Biol.Chem.261，10871-10878）。在本文中，我们描述了这些蛋白质的另一个，称为大鼠催乳素样蛋白A（rPLP-A），因为它的同源性与催乳素的cDNA克隆的分离和表征。对应于rPLP-A的单个mRNA转录物的长度为1千碱基，并且在妊娠第14天首次出现，比rPLII mRNA晚2天，然后增加并保持在高水平直到足月。与rPLP-A cDNA克隆杂交的mRNA在体外翻译成25，000道尔顿的蛋白质，其被狗胰腺微粒体加工成27，000道尔顿。从rPLP-A克隆的核苷酸序列推导的氨基酸序列表明，rPLP-A是具有两个潜在糖基化信号的196个氨基酸的分泌蛋白。该序列在氨基酸水平上与大鼠和人催乳素和rPLII的同源性超过40%。这项研究提供了明确的证据，存在大量的未描述的催乳素样蛋白在晚期大鼠胎盘。
The mid- to late-term rat placenta produces several moderately abundant proteins in a specific temporal manner, one of which we have identified as rat placental lactogen II (rPLII) (Duckworth, M. L., Kirk, K. L., and Friesen, H. G. (1986) J. Biol. Chem. 261, 10871-10878). In this paper, we describe the isolation and characterization of cDNA clones of another of these proteins, called rat prolactin-like protein A (rPLP-A) because of its homology with the prolactins. The single mRNA transcript corresponding to rPLP-A is 1 kilo-base in length and first appears at Day 14 of pregnancy, 2 days later than rPLII mRNA, and then increases and remains at high levels until term. The mRNA hybridizing to rPLP-A cDNA clones translates in vitro to a protein of 25,000 daltons which is processed by dog pancreatic microsomes to 27,000 daltons. The amino acid sequence deduced from the nucleotide sequence of the rPLP-A clones suggests that rPLP-A is a secreted protein of 196 amino acids with two potential glycosylation signals. The sequence is more than 40% homologous at the amino acid level to rat and human prolactins and rPLII. This study provides clear evidence for the existence of considerable amounts of undescribed prolactin-like proteins in late term rat placenta.