Bacteriophage phi297, a new species of Pseudomonas aeruginosa temperate phages with a mosaic genome: Potential use in phage therapy
Bacteriophage phi297, a new species of Pseudomonas aeruginosa temperate phages with a mosaic genome: Potential use in phage therapy
复制标题
DOI：
10.1134/s102279541106007x
复制
发表时间：
2011-07-01
影响因子：
0.6
通讯作者：
Krylov, V. N.
中科院分区：
文献类型：
--
作者：
Bourkal'tseva, M. V.;Krylov, S. V.;Krylov, V. N.
研究了嗜盐性温和铜绿假单胞菌噬菌体phi297的基因组及其突变株的裂解活性。phi297基因组全序列测定结果表明，phi297基因组具有嵌合结构。phi297基因组与F116和F11D3基因组部分同源。根据阴性菌落的形态学特征，假定温和铜绿假单胞菌噬菌体phi297具有高裂解活性。分离出能够裂解野生型噬菌体细菌的毒性突变体phi297vir。比较phi297和来自两个制造商（下诺夫哥罗德和彼尔姆的设施）的商业混合物的Phi297的裂解活性。噬菌体phi297引起对来自商业制备物的青霉素抗性的突变PAO 1细菌的裂解，但phi297的裂解活性谱比商业青霉素的谱窄。某些温和噬菌体的非回复毒性突变体被提议用于治疗铜绿假单胞菌感染。
The genome of halo-forming temperate Pseudomonas aeruginosa phage phi297 and lytic activity of its virulent mutant were studied. A mosaic structure was revealed for phi297 genome by its complete sequencing. The phi297 genome was partly homologous to the genomes of phages D3 and F116. High lytic activity was assumed for temperate P. aeruginosa bacteriophage phi297 on the basis of morphological features of negative colonies. Virulent mutant phi297vir, which was capable of lysing the wild-type phage bacteria, was isolated. Lytic activity was compared for phi297 and the phages from commercial mixtures of two manufacturers (facilities of Nizhnii Novgorod and Perm'). Phage phi297 caused lysis of the mutant PAO1 bacteria that were resistant to the phages from commercial preparations, but the lytic activity spectrum of phi297 was narrower that the spectra of the commercial phages. The use of nonreverting virulent mutants of certain temperate bacteriophages was proposed for the treatment of P. aeruginosa infections.