ARS BINDING FACTOR-I BINDS ADJACENT TO RAP1 AT THE UASS OF THE YEAST GLYCOLYTIC GENES PGK AND PYK1
ARS BINDING FACTOR-I BINDS ADJACENT TO RAP1 AT THE UASS OF THE YEAST GLYCOLYTIC GENES PGK AND PYK1
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DOI：
10.1093/nar/18.18.5393
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发表时间：
1990-09-25
影响因子：
14.9
通讯作者：
KINGSMAN, SM
中科院分区：
文献类型：
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作者：
CHAMBERS, A;STANWAY, C;KINGSMAN, SM
编码糖酵解酶磷酸甘油酸激酶（PGK）的酵母基因的UAS包含几个参与转录激活的不同序列元件。这些元件包括与RAP 1蛋白结合的激活剂核心序列和五聚体序列5 ″ CTTCC 3 ″的三个拷贝。激活子核心序列的上游是CTTCC 3“。激活子核心序列的上游是一个区域（Yfp），被鉴定为强DNA-蛋白质相互作用的位点。Yfp区域包含因子ABF 1的共有结合位点。我们已经将结合Yfp区域的Y蛋白纯化至均一。Y蛋白在SDS-聚丙烯酰胺凝胶电泳上以双峰形式迁移，表观分子量为125 KDa。这些特性与ABF 1相似。体外合成的ABF 1与Yfp区域强烈结合，并形成与Y蛋白形成的复合物具有相同迁移率的凝胶阻滞复合物。丙酮酸激酶基因（PYK 1）启动子的UAS 1含有RAP 1结合位点和单拷贝的CTTCC序列。我们现在已经确定了一个ABF 1结合位点接近RAP 1结合位点和CTTCC序列的PYK 1启动子。因此，PGK和PYK 1 UAS的组织彼此相似，并且与也含有这些序列的转录沉默子HMR（E）相似。
The UAS of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) contains several different sequence elements involved in transcriptional activation. These elements include the activator core sequence, which is bound by the RAP 1 protein, and three copies of the pentamer sequence 5'' CTTCC 3''. Upstream of the activator core sequence is CTTCC 3''. Upstream of the activator core sequence is a region (Yfp), identified as the site of a strong DNA-protein interaction. The Yfp region contains the consensus binding site for the factor ABF1. We have purified the Y protein, which binds to the Yfp region, to homogeneity. The Y protein migrates as a doublet on SDS-polyacrylamide gel electrophoresis with an apparent molecular weight of 125 KDa. These properties are similar to those of ABF1. ABF1 synthesized in vitro bound strongly to the Yfp region and formed a gel retardation complex of identical mobility to the complex formed by the Y protein. UAS1 of the pyruvate kinase gene (PYK1) promoter contains a RAP1 binding site and single copy of the CTTCC sequence. We have now identified an ABF1 binding site close to the RAP1 binding site and CTTCC sequence in the PYK1 promoter. The organisation of the PGK and PYK1 UASs is thus similar to each other and to the transcriptional silencer HMR(E) which also contains these sequences.