Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses
Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses
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DOI：
10.1111/j.1469-8137.2010.03535.x
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发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
Nakazono, Mikio
中科院分区：
文献类型：
--
作者：
Rajhi, Imene;Yamauchi, Takaki;Nakazono, Mikio
为了适应土壤淹水，一些禾本科植物，如玉米(Zea Mays)，在根皮层形成溶生性的通风组织。在内涝过程中积累的乙烯促进了通风组织的形成。本研究的目的是寻找在玉米根中表达的与通风组织形成相关的基因，为理解通风组织形成的分子机制奠定基础。玉米植株生长在淹水条件下，有或没有乙烯感受抑制剂1-甲基环丙烯(1-MCP)的预处理，或在好氧条件下。用激光显微切割分离大脑皮层细胞，用微阵列技术检测其mRNA水平，芯片分析发现575个基因在淹水条件下表达上调或下调，其诱导或抑制可被1-MCP抑制，差异表达基因包括与产生或清除活性氧有关的基因、钙信号转导以及细胞壁松弛和降解相关基因。这项研究的结果将有助于更好地理解根溶生通风组织的形成机制。
P>To adapt to waterlogging in soil, some gramineous plants, such as maize (Zea mays), form lysigenous aerenchyma in the root cortex. Ethylene, which is accumulated during waterlogging, promotes aerenchyma formation. However, the molecular mechanism of aerenchyma formation is not understood.The aim of this study was to identify aerenchyma formation-associated genes expressed in maize roots as a basis for understanding the molecular mechanism of aerenchyma formation. Maize plants were grown under waterlogged conditions, with or without pretreatment with an ethylene perception inhibitor 1-methylcyclopropene (1-MCP), or under aerobic conditions. Cortical cells were isolated by laser microdissection and their mRNA levels were examined with a microarray.The microarray analysis revealed 575 genes in the cortical cells, whose expression was either up-regulated or down-regulated under waterlogged conditions and whose induction or repression was suppressed by pretreatment with 1-MCP.The differentially expressed genes included genes related to the generation or scavenging of reactive oxygen species, Ca2+ signaling, and cell wall loosening and degradation. The results of this study should lead to a better understanding of the mechanism of root lysigenous aerenchyma formation.