Influence of the olfactory placode on the development of the brain in Xenopus laevis (Daudin) I. Axonal growth and connections of the transplanted olfactory placode
Influence of the olfactory placode on the development of the brain in Xenopus laevis (Daudin) I. Axonal growth and connections of the transplanted olfactory placode
复制标题
嗅板对非洲爪蟾 (Daudin) 大脑发育的影响 I. 移植嗅板的轴突生长和连接
DOI：
10.1016/0306-4522(80)90134-7
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发表时间：
1980
期刊：
影响因子：
3.3
通讯作者：
P. Graziadei
中科院分区：
文献类型：
--
作者：
R. Stout;P. Graziadei
将不同发育阶段的非洲爪蟾（xenopus laevisis幼虫）的嗅觉基因提取并移植到寄主幼虫的头部，以确定供体/寄主年龄对移植存活率的影响。当供体和宿主均处于29/30期时，移植物的生存能力（定义为移植物的神经在宿主中枢神经系统中的渗透）达到最大。利用29/30期的幼虫，在宿主的头部绘制了三个不同的区域，移植的嗅觉基因持续地将其神经分别发送到大脑的三个不同区域，即端脑、间脑和髓脑。额外的嗅觉输入在穿透端脑的地方引起了肥厚。当嗅觉输入穿透间脑时，在丘脑背侧观察到神经元成分的显著增生和肾小球的形成。当输入到达这一区域时，髓脑的组织没有发生总体变化。包括第41期在内的捐赠者重塑了基因座。在这一阶段后切除基板会导致器官的永久性丧失以及嗅球和端脑发育不全。结果表明，嗅觉感觉神经元具有独特的特性，可以诱导宿主中枢神经系统异常区域的增生，并在这些区域确定通常被描述为肾小球的特征性末端结构。
The olfactory placode ofXenopus laevislarvae at different stages of development has been removed and transplanted onto the head of host larvae to determine the effect of the donor/host age on the viability of the transplant. Viability of the transplant (defined as penetration of the nerve of the transplanted placode in the host's CNS) has been found to be maximal when both donor and host were at about stage29/30. Using stage 29/30 larvae, three distinct zones have been mapped on the host's head from which the transplanted olfactory placode consistently sent its nerve to three different regions of the brain, namely to the telencephalon, the diencephalon and to the myelencephalon, respectively. The additional olfactory input induced a hypertrophy where it penetrated the telencephalon. When the olfactory input penetrated the diencephalon a marked hyperplasia of the neuronal elements and formation of glomeruli was observed in the dorsal thalamus. There was no overall change in the organization of the myelencephalon when the input reached this region. Donors up to and including stage 41 remade the placode. Removal of the placode after this stage resulted in permanent loss of the organ and hypoplasia of the olfactory bulb and telencephalon.The results illustrate the unique property of the olfactory sensory neurons to induce hyperplasia in anomalous regions of the host's CNS and to determine, in these regions, the characteristic terminal structures which are commonly described as glomeruli.