Differential control of somatostatin messenger RNA in rat gastric corpus and antrum. Role of acid, food, and capsaicin-sensitive afferent neurons.
Differential control of somatostatin messenger RNA in rat gastric corpus and antrum. Role of acid, food, and capsaicin-sensitive afferent neurons.
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大鼠胃体和胃窦生长抑素信使 RNA 的差异控制。
DOI：
10.1172/jci116177
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
G. Dockray
中科院分区：
文献类型：
--
作者：
A. Sandvik;R. Dimaline;E. Forster;D. Evans;G. Dockray
采用聚合酶链反应产生的探针，采用Northern和slot印迹法对大鼠胃窦和胃体中的生长抑素信使RNA进行定量分析。禁食48小时可提高幽门胃窦区生长抑素mRNA的丰度，而胃泌酸区则没有。在禁食大鼠中，奥美拉唑抑制胃酸分泌可降低胃窦中生长抑素mRNA的表达，而体中没有。相比之下，辣椒素对小直径传入损伤的大鼠，体部而非胃窦的生长抑素mRNA丰度显著降低。辣椒素的作用不能归因于胃内分泌细胞基因表达的非特异性变化，因为组氨酸脱羧酶mRNA（一种不同胃内分泌细胞类型的功能调节标记物）的丰度没有随着辣椒素而改变。胃辣椒素敏感事件中含有丰富的降钙素基因相关肽，对该肽有抗体的大鼠体内生长抑素mRNA减少。此外，在对照组大鼠中输注降钙素基因相关肽可显著增加胃体中生长抑素mRNA的表达。结果表明，生长抑素mRNA丰度受胃腔内容物和外源性传入神经支配，但这些因素在胃窦和胃体中的相对重要性不同：胃腔内容物在胃窦和胃原发传入中相对更重要，而胃原发传入则使用降钙素基因相关肽。
Somatostatin messenger RNA in the antrum and corpus of rat stomach was quantified by Northern and slot blotting using a probe generated by the polymerase chain reaction. Fasting for 48 h enhanced the abundance of somatostatin mRNA in the pyloric antral region, but not in the acid-secreting region of the stomach. In fasted rats, somatostatin mRNA in antrum, but not corpus, was decreased by inhibition of acid secretion with omeprazole. In contrast, in rats treated with capsaicin to lesion small diameter afferents there was a significant decrease in somatostatin mRNA abundance in the corpus but not antrum. The effects of capsaicin cannot be attributed to nonspecific changes in gastric endocrine cell gene expression, since the abundance of histidine decarboxylase mRNA (which is a functionally regulated marker for a different gastric endocrine cell type) did not change with capsaicin. Gastric capsaicin-sensitive afferents are rich in calcitonin gene-related peptide, and in rats with antibodies to this peptide there was reduced corpus somatostatin mRNA. Moreover, infusion of calcitonin gene-related peptide in control rats produced a significant increase in somatostatin mRNA in the gastric corpus. The results indicate that somatostatin mRNA abundance is controlled by the gastric luminal contents and the extrinsic afferent innervation, but the relative importance of these factors differs in antrum and corpus: luminal contents are relatively more important in antrum and primary afferents using calcitonin gene-related peptide in the corpus.