Glucose repression affects ion homeostasis in yeast through the regulation of the stress-activated ENA1 gene
Glucose repression affects ion homeostasis in yeast through the regulation of the stress-activated ENA1 gene
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DOI：
10.1046/j.1365-2958.1997.5531917.x
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发表时间：
1997-10-01
影响因子：
3.6
通讯作者：
Estruch, F
中科院分区：
文献类型：
--
作者：
Alepuz, PM;Cunningham, KW;Estruch, F
在这份报告中，我们表明，ENA 1/PMR 2A基因是在葡萄糖阻遏。SNF 1蛋白激酶独立于HOG和钙调神经磷酸酶途径发挥作用，对从葡萄糖抑制中释放ENA 1至关重要。转录抑制因子Ssn 6p负调控ENA 1的表达，与其他葡萄糖抑制基因一样，这种抑制部分由Mig 1 p介导。从ENA 1启动子中删除包括两个Mig 1 p共有结合位点的片段，在没有添加盐的情况下在葡萄糖中获得高水平的表达。我们认为SNF 1途径对ENA 1的调节可能是酵母细胞通过激活针对不同类型压力的保护系统来响应碳源饥饿的一般机制的一部分。
In this report we show that the ENA1/PMR2A gene is under glucose repression. The SNF1 protein kinase, acting independently from the HOG and calcineurin pathways, is essential to release ENA1 from glucose repression. The transcriptional repressor Ssn6p negatively regulates ENA1 expression and, like other glucose repressible genes, this repression is mediated in part by Mig1p. Deletion of a fragment from the ENA1 promoter that includes two Mig1p consensus binding sites gives a high level of expression in glucose without added salt. We suggest that regulation of ENA1 by the SNF1 pathway could be part of a general mechanism through which yeast cells respond to carbon source starvation by activating protective systems against different types of stress.