Spatiotemporal functional organization of excitatory synaptic inputs onto macaque V1 neurons
Spatiotemporal functional organization of excitatory synaptic inputs onto macaque V1 neurons
复制标题
猕猴 V1 神经元兴奋性突触输入的时空功能组织
DOI：
10.1101/558163
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Tang, Shiming
中科院分区：
文献类型：
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作者：
Ju, Niansheng;Li, Yang;Liu, Fang;Jiang, Hongfei;Macknik, Stephen;Martinez-Conde, Susana;Tang, Shiming
突触输入到树突上的整合为神经元计算提供了基础。虽然最近的研究已经开始概述单个神经元上突触输入的空间组织，但与特定神经功能相关的基本原理尚未得到很好的理解。在这里，我们进行双光子树突状成像与基因编码的谷氨酸传感器在清醒的猴子，并映射兴奋性突触输入的树突上的个别V1浅层神经元具有高的空间和时间分辨率。我们发现一个功能的整合和权衡之间的方向选择性和颜色选择性的输入在基底树突的个别V1神经元。树突上的突触输入在空间上被刺激特征聚集，但在功能上分散在多维特征空间中，为树突分支上的局部特征整合提供了潜在的基底。此外，顶端树突输入有更大的感受野和更长的反应潜伏期比基底树突输入，表明顶端树突在整合反馈在视觉信息处理中的主导作用。
The integration of synaptic inputs onto dendrites provides the basis for neuronal computation. Whereas recent studies have begun to outline the spatial organization of synaptic inputs on individual neurons, the underlying principles related to the specific neural functions are not well understood. Here we perform two-photon dendritic imaging with a genetically-encoded glutamate sensor in awake monkeys, and map the excitatory synaptic inputs on dendrites of individual V1 superficial layer neurons with high spatial and temporal resolution. We find a functional integration and trade-off between orientation-selective and color-selective inputs in basal dendrites of individual V1 neurons. Synaptic inputs on dendrites are spatially clustered by stimulus feature, but functionally scattered in multidimensional feature space, providing a potential substrate of local feature integration on dendritic branches. Furthermore, apical dendrite inputs have larger receptive fields and longer response latencies than basal dendrite inputs, suggesting a dominant role for apical dendrites in integrating feedback in visual information processing.