RAT MODEL OF ARTERIAL THROMBOSIS INDUCED BY FERRIC-CHLORIDE
RAT MODEL OF ARTERIAL THROMBOSIS INDUCED BY FERRIC-CHLORIDE
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DOI：
10.1016/0049-3848(90)90106-m
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发表时间：
1990-11-15
影响因子：
7.5
通讯作者：
SANDUSKY, GE
中科院分区：
文献类型：
--
作者：
KURZ, KD;MAIN, BW;SANDUSKY, GE
这些研究的目的是建立动脉血栓形成的小动物模型，用于研究新型抗血栓药物，验证闭塞性血栓形成的简单温度指数，并描述血栓的成分。小型热电偶传感器由现成的材料制成。将热电偶插入麻醉大鼠的颈动脉下方并连续记录血管温度。通过将 FeCl3 溶液局部涂抹到热电偶上方的动脉来诱导动脉损伤。为了验证血栓闭塞与血管温度之间的关系，同时记录血流速度、损伤近端和温度。当速度平均为 24 +/- 时，温度迅速下降。 12% 的控制力和速度在 20 秒内与零没有差别。在正常血管中，平均流速相对于控制并没有显着降低，直到固定狭窄使直径减少 78%。由温度突变表示的平均闭塞时间 (TTO) 范围为 56 .+-。 4 分钟至 14 点。+-。分别施用 10% 和 65% FeCl3 后 1 分钟。在暴露于 FeCl3 后的不同时间固定血管片段并通过扫描电子显微镜检查。观察到内皮损伤，并与由活化的血小板、纤维蛋白链和截留的红细胞组成的血栓相关。结果表明，FeCl3 剂量依赖性地诱导闭塞性混合血栓的形成，通过监测大鼠颈动脉中使用 FeCl3 和温度快速下降之间的时间来指示闭塞性混合血栓的形成。
The purposes of these studies were to produce a small animal model of arterial thrombosis for study of novel antithrombotic agents, to validate a simple temperature index of occlusive thrombosis, and to describe the composition of the thrombus. Small thermocouple transducers were fabricated from readily available materials. A thermocouple was inserted under a carotid artery of the anesthetized rat and vessel temperature was recorded continuously. Arterial injury was induced by FeCl3 solution applied topically to the artery above the thermocouple. To validate the relationship between thrombotic occlusion and vessel temperature, blood flow velocity, proximal to the injury, and temperature were recorded simultaneously. Temperature decreased rapidly when velocity averaged 24 .+-. 12 percent of control and velocity did not differ from zero within 20 sec. In normal vessels, average flow velocity did not decrease significantly fron control until a fixed stenosis decreased diameter by 78 percent. Average time to occlusion (TTO), signaled by the abrupt temperature inflection, ranged from 56 .+-. 4 min to 14 .+-. 1 min after 10 and 65 percent FeCl3 application respectively. Vessel segments were fixed at various times after FeCl3 exposure and examined by scanning electron microscopy. Endothelial damage was observed and was associated with thrombus composed of activated platelets, fibrin strands and entrapped erythrocytes. The results demonstrate that FeCl3 dose-dependently induced formation of an occlusive mixed thrombus that was indexed by monitoring the time between FeCl3 application and a rapid temperature decrease in the carotid artery of the rat.