A novel method for quantifying traction forces on hexagonal micropatterned protein features on deformable poly-dimethyl siloxane sheets
A novel method for quantifying traction forces on hexagonal micropatterned protein features on deformable poly-dimethyl siloxane sheets
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DOI：
10.1016/j.mex.2019.05.011
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发表时间：
2019-01-01
期刊：
影响因子：
1.9
通讯作者：
Lemmon, Christopher A.
中科院分区：
文献类型：
--
作者：
Griffin, Brian P.;Largaespada, Christopher J.;Lemmon, Christopher A.
存在许多量化细胞牵引力的方法，包括牵引力显微镜和微制造柱阵列。然而，这些方法有局限性，包括需要移除细胞以确定未偏转的颗粒位置，以及无法分别量化具有低细胞骨架刚度的细胞的力。在这里，我们提出了一种新的牵引力量化方法，消除了这两个限制。通过使用微接触印刷蛋白点的六角形图案，一种新的计算算法和聚二甲基硅氧烷（PDMS）共混物的薄表面，我们展示了一个系统：在稳定且易于制造的均匀表面上量化细胞力。利用蛋白质点的六角形图案和计算几何来量化细胞力，而不需要细胞去除。量化具有低细胞骨架刚性的细胞的细胞力。(C) 2019作者。Elsevier B.V.出版
Many methods exist for quantifying cellular traction forces, including traction force microscopy and microfabricated post arrays. However, these methodologies have limitations, including a requirement to remove cells to determine undeflected particle locations and the inability to quantify forces of cells with low cytoskeletal stiffness, respectively. Here we present a novel method of traction force quantification that eliminates both of these limitations. Through the use of a hexagonal pattern of microcontact-printed protein spots, a novel computational algorithm, and thin surfaces of polydimethyl siloxane (PDMS) blends, we demonstrate a system that:quantifies cellular forces on a homogeneous surface that is stable and easily manufactured.utilizes hexagonal patterns of protein spots and computational geometry to quantify cellular forces without need for cell removal.quantifies cellular forces in cells with low cytoskeletal rigidity. (C) 2019 The Author(s). Published by Elsevier B.V.