SMALL INHIBITORY CEREBELLAR INTERNEURONS GROW IN A PERPENDICULAR ORIENTATION TO GRANULE CELL NEURITES IN CULTURE
SMALL INHIBITORY CEREBELLAR INTERNEURONS GROW IN A PERPENDICULAR ORIENTATION TO GRANULE CELL NEURITES IN CULTURE
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DOI：
10.1016/0896-6273(89)90178-5
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发表时间：
1989-02-01
期刊：
影响因子：
16.2
通讯作者：
SCHACHNER, M
中科院分区：
文献类型：
--
作者：
HEKMAT, A;KUNEMUND, V;SCHACHNER, M
当将来自出生后早期小鼠小脑的小神经元的外植体或再聚集体接种在层粘连蛋白和聚-D-赖氨酸的混合物上时，观察到小细胞的突起的方向在几天后基本上垂直于颗粒细胞神经突起的方向。这些细胞首先具有双极形态，然后在长而细的轴突样突起的对面精心制作丰富的树突状树状结构。几条证据表明，这些细胞是小脑皮质的小抑制性中间神经元：它们摄取CABA，表达高水平的N-CAM的胚胎形式，不表达11，少突胶质细胞标记物04，或胶质细胞标记物波形蛋白，并显示超微结构特征，让人想起体内的星状细胞和/或篮状细胞。这些观察结果表明，可以在培养中研究小抑制性中间神经元的定向定位的详细说明，从而提供了阐明特定神经细胞相对于其他神经细胞定向的细胞和分子机制的可能性。
When explants or reaggregates of small neurons from early postnatal mouse cerebella are plated on a mixture of laminin and poly-D-lysine, one observes small cells with an orientation of processes largely perpendicular to the direction of granule cell neurites after several days. These cells first have a bipolar morphology and then elaborate a rich dendritic arbor-like structure opposite a long, thin axon-like process. Several lines of evidence suggest that these cells are the small inhibitory interneurons of the cerebellar cortex: They take up CABA, express high levels of the embryonic form of N-CAM, do not express 11, the oligodendrocyte marker 04, or the glial marker vimentin, and display ultrastructural features reminiscent of stellate and/or basket cells in vivo. These observations suggest that the elaboration of directional positioning of small inhibitory interneurons can be studied in culture, thus offering the possibility to elucidate the cellular and molecular mechanisms underlying the orientation of particular neural cells with regard to others.