Identification of Pns6, a putative movement protein of RRSV, as a silencing suppressor.
Identification of Pns6, a putative movement protein of RRSV, as a silencing suppressor.
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DOI：
10.1186/1743-422x-7-335
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发表时间：
2010-11-22
期刊：
影响因子：
4.8
通讯作者：
Xie L
中科院分区：
文献类型：
--
作者：
Wu J;Du Z;Wang C;Cai L;Hu M;Lin Q;Wu Z;Li Y;Xie L
RNA沉默是植物中有效的抗病毒反应。作为一种防御手段，大多数植物和一些动物病毒编码RNA沉默抑制子。在本研究中，我们发现，Pns6，一个假定的运动蛋白的水稻参差不齐的矮化病毒（RRSV），表现出沉默抑制活性的共浸润试验与报告绿色荧光蛋白（GFP）在转基因烟草本塞姆亚纳线16 c。RRSV的Pns6抑制正义RNA诱导的局部沉默，但对dsRNA诱导的局部沉默没有影响。参与RNA结合的区域的缺失废除了Pns6的沉默抑制活性。此外，Pns6的表达增强了马铃薯病毒×在N.本萨米亚纳总的来说，这些结果表明RRSV Pns6作为RNA沉默的病毒抑制子发挥作用，其靶向RNA沉默途径中dsRNA形成的上游步骤。这是第一个沉默抑制基因被确定从水稻病毒属。
RNA silencing is a potent antiviral response in plants. As a counterdefense, most plant and some animal viruses encode RNA silencing suppressors. In this study, we showed that Pns6, a putative movement protein of Rice ragged stunt virus (RRSV), exhibited silencing suppressor activity in coinfiltration assays with the reporter green fluorescent protein (GFP) in transgenic Nicotiana benthamiana line 16c. Pns6 of RRSV suppressed local silencing induced by sense RNA but had no effect on that induced by dsRNA. Deletion of a region involved in RNA binding abolished the silencing suppressor activity of Pns6. Further, expression of Pns6 enhanced Potato virus × pathogenicity in N. benthamiana. Collectively, these results suggested that RRSV Pns6 functions as a virus suppressor of RNA silencing that targets an upstream step of the dsRNA formation in the RNA silencing pathway. This is the first silencing suppressor to be identified from the genus Oryzavirus.