Analysis of human hepatic microsomal glucose-6-phosphatase in clinical conditions where the T2 pyrophosphate/phosphate transport protein is absent.
Analysis of human hepatic microsomal glucose-6-phosphatase in clinical conditions where the T2 pyrophosphate/phosphate transport protein is absent.
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在 T2 焦磷酸盐/磷酸盐转运蛋白缺失的临床条件下对人肝微粒体葡萄糖 6 磷酸酶进行分析。
DOI：
10.1042/bj2810859
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Burchell,A
中科院分区：
文献类型：
--
作者：
Nordlie,RC;Scott,HM;Waddell,ID;Hume,R;Burchell,A
一组罕见的人肝微粒体（其中葡萄糖-6-磷酸酶系统的焦磷酸盐/磷酸盐转运蛋白T2在免疫学上完全不存在）的可用性允许进一步表征多组分葡萄糖-6-磷酸酶（EC 3.1.3.9）。发现完整微粒体中的焦磷酸酶活性完全不存在，但在破坏的微粒体中正常。然而，当葡萄糖6-磷酸是底物时，Pi没有在微粒体的管腔内积累。如果内质网中只有一种转运蛋白能够将葡萄糖-6-磷酸酶产生的Pi转运出腔，则这与预测的结果不同。结果表明，人肝内质网的焦磷酸盐/磷酸盐转运系统必须比以前认为的更复杂，因为它必须包含至少两种蛋白质组分。
The availability of a rare set of human hepatic microsomes in which T2, a pyrophosphate/phosphate transport protein of the glucose-6-phosphatase system, has been shown immunologically to be completely absent, has permitted further characterization of multicomponent glucose-6-phosphatase (EC 3.1.3.9). Pyrophosphatase activity in intact microsomes was found to be totally absent, but was normal in disrupted microsomes. However, Pi did not accumulate within the lumen of the microsomes when glucose 6-phosphate was the substrate. This was not as predicted if there is only one transport protein in the endoplasmic reticulum capable of transporting Pi, produced by glucose-6-phosphatase, out of the lumen. The results suggest that the pyrophosphate/phosphate transport system of human hepatic endoplasmic reticulum must be more complex than previously thought, as it must comprise at least two protein components.
胎儿时期人体外部尺寸的发育和生长
影响因子：
2.5
作者：
R. Scammon;L. A. Calkins
通讯作者：
L. A. Calkins