SEQUENCING AND THE ALIGNMENT OF STRUCTURAL GENES IN THE NQR OPERON ENCODING THE NA+-TRANSLOCATING NADH-QUINONE REDUCTASE FROM VIBRIO-ALGINOLYTICUS
SEQUENCING AND THE ALIGNMENT OF STRUCTURAL GENES IN THE NQR OPERON ENCODING THE NA+-TRANSLOCATING NADH-QUINONE REDUCTASE FROM VIBRIO-ALGINOLYTICUS
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DOI：
10.1016/0014-5793(95)00283-f
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发表时间：
1995-04-17
期刊：
影响因子：
3.5
通讯作者：
UNEMOTO, T
中科院分区：
文献类型：
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作者：
HAYASHI, M;HIRAI, K;UNEMOTO, T
我们先前克隆了来自海洋溶藻弧菌的编码Na+易位NADH-醌还原酶（NQR）的nqr操纵子的一部分[Hayashi et al.，FEES Lett，356（1994）330-332]，从其核苷酸序列中，识别出编码γ-亚基（27.7 kDa）的四个连续开放阅读框（ORF）、22.6 kDa和21.5 kDa的两个未鉴定的ORF以及β-亚基（45.3 kDa）。编码α-亚基的基因位于上游，并且与Beattie等人的最近报道[FEES Lett，356（1994）333-338]一起，发现nqr操纵子由六个连续的结构基因构建，其中nqr 1、nqr 3和nqr 6分别对应于NQR复合物的α-、γ-和β-亚基。
We previously cloned a part of nqr operon encoding the Na+-translocating NADH-quinone reductase (NQR) from the marine Vibrio alginolyticus [Hayashi et al., FEES Lett, 356 (1994) 330-332], From its nucleotide sequences, four consecutive open reading frames (ORF) encoding the gamma-subunit (27.7 kDa), two unidentified ORFs of 22.6 kDa and 21.5 kDa, and the beta-subunit (45.3 kDa) were recognized. The gene encoding the alpha-subunit was located upstream, and together with the recent report by Beattie et al. [FEES Lett, 356 (1994) 333-338], the nqr operon was found to be constructed from six consecutive structural genes, where nqr1, nqr3 and nqr6 correspond to the alpha-, gamma-, and beta-subunits, respectively, of the NQR complex.