Comparison of alcohol dehydrogenase expression in Drosophila melanogaster and D. simulans.
Comparison of alcohol dehydrogenase expression in Drosophila melanogaster and D. simulans.
复制标题
黑腹果蝇和模拟果蝇中乙醇脱氢酶表达的比较。
DOI：
10.1093/oxfordjournals.molbev.a040630
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发表时间：
1991
影响因子：
10.7
通讯作者：
Laurie,CC
中科院分区：
文献类型：
--
作者：
Thomson,MS;Jacobson,JW;Laurie,CC
对黑腹果蝇（Drosophilamelanogaster）及其近缘种D. simulans。ADH活性，ADH交叉反应物质（CRM），和ADH-mRNA的水平进行了分析的几个菌株的每一个物种，来自世界各地的不同地理位置。在所有发育阶段，种内菌株间ADH活性、CRM水平和RNA水平存在相当大的数量变化。然而，这两个物种之间唯一一致的差异是蛹RNA水平和成虫后期活动和CRM水平。晚成黑腹果蝇慢等位酶纯合子的ADH活性和CRM的水平大约是simulans苍蝇的两倍。CRM上的活性随菌株的回归是高度显著的，并且对于每个物种基本上相同，这意味着物种之间的大部分（如果不是全部）活性差异是由于ADH蛋白浓度的差异。相比之下，有CRM水平上的mRNA水平的任何物种的成年人没有显着的回归，也没有显着差异的RNA水平之间的物种。因此，ADH蛋白浓度的差异不是由于RNA模板的可用性。因此，种间差异ADH水平在成人必须是由于在翻译的两种RNA的速率或蛋白质的稳定性差异的差异。
Alcohol dehydrogenase (ADH) gene expression was analyzed in Drosophila melanogaster and its sibling species D. simulans. The levels of ADH activity, ADH-cross-reacting material (CRM), and ADH-mRNA were analyzed for several strains of each species, which derive from diverse geographic locations around the world. There is considerable quantitative variation in ADH activity, CRM level, and RNA level among strains within species at all developmental stages. However, the only consistent differences between the two species are in pupal RNA level and in late-adult activity and CRM level. Late-adult melanogaster flies that are homozygous for the Slow allozyme have approximately twice the level of ADH activity and CRM as do simulans flies. The regression of activity on CRM over strains is highly significant and essentially the same for each species, which means that most, if not all, of the activity difference between the species is due to a difference in concentration of the ADH protein. In contrast, there is no significant regression of CRM level on mRNA level in adults of either species; nor is there a significant difference in RNA level between species. Therefore, the difference in ADH protein concentration is not due to RNA template availability. Thus, the interspecific difference in ADH level in adults must be due either to a difference in the rate of translation of the two RNAs or to a difference in protein stability.