Two-photon excitation STED microscopy
Two-photon excitation STED microscopy
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DOI：
10.1364/oe.17.014567
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发表时间：
2009-08-17
期刊：
影响因子：
3.8
通讯作者：
Hell, Stefan W.
中科院分区：
文献类型：
--
作者：
Moneron, Gael;Hell, Stefan W.
We report sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy achieved by merging this technique with stimulated-emission depletion (STED). We demonstrate an easy-to-implement and promising laser combination based on a short-pulse laser source for two-photon excitation and a continuous-wave (CW) laser source for resolution enhancement. Images of fluorescent nanoparticles and the immunostained transcription regulator NF kappa B in mammalian cell nuclei exhibit resolutions of
