Hydrogen sulfide alleviates the aluminum-induced changes in Brassica napus as revealed by physiochemical and ultrastructural study of plant
Hydrogen sulfide alleviates the aluminum-induced changes in Brassica napus as revealed by physiochemical and ultrastructural study of plant
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植物理化和超微结构研究表明，硫化氢可减轻铝诱导的甘蓝型油菜变化
DOI：
10.1007/s11356-014-3551-y
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发表时间：
2015-02-01
影响因子：
5.8
通讯作者：
Zhou, Weijun
中科院分区：
文献类型：
--
作者：
Ali, Basharat;Qian, Ping;Zhou, Weijun
本文研究了硫化氢（H2S）对油菜（Brassica napus L.）在溶液培养条件下，在三种水平（0、0.1和0.3mM）的铝（Al）下，在施用或不施用H2S供体硫氢化钠（NaHS）（0.3mM）的情况下，研究结果表明，H2S处理显著促进了B的生长、光合气体交换以及叶片和根系的养分含量。铝胁迫下的油菜植株。外源H2S处理显著降低了铝胁迫下植物不同部位的铝浓度，并通过提高叶片和根系抗氧化酶活性，减少了丙二醛和活性氧的产生。此外，本研究表明，外源H2S改善细胞结构，并显示出干净的叶肉和根尖细胞。在显微镜下可观察到叶绿体具有发达的类囊体膜。在H2S和Al的联合作用下，根尖细胞中的线粒体、内质网和高尔基体发生了一些变化。说明外源H2S处理改善了Al胁迫下B的生长、光合参数、元素含量以及叶片和根系的生化和超微结构变化。油菜。
In the present study, ameliorating role of hydrogen sulfide (H2S) in oilseed rape (Brassica napus L.) was studied with or without application of H2S donor sodium hydrosulfide (NaHS) (0.3 mM) in hydroponic conditions under three levels (0, 0.1 and 0.3 mM) of aluminum (Al). Results showed that addition of H2S significantly improved the plant growth, photosynthetic gas exchange, and nutrients concentration in the leaves and roots of B. napus plants under Al stress. Exogenously applied H2S significantly lowered the Al concentration in different plant parts, and reduced the production of malondialdehyde and reactive oxygen species by improving antioxidant enzyme activities in the leaves and roots under Al stress. Moreover, the present study indicated that exogenously applied H2S improved the cell structure and displayed clean mesophyll and root tip cells. The chloroplast with well-developed thylakoid membranes could be observed in the micrographs. Under the combined application of H2S and Al, a number of modifications could be observed in root tip cell, such as mitochondria, endoplasmic reticulum, and golgi bodies. Thus, it can be concluded that exogenous application of H2S under Al stress improved the plant growth, photosynthetic parameters, elements concentration, and biochemical and ultrastructural changes in leaves and roots of B. napus.