The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells
The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells
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DOI：
10.1016/j.femsim.2004.03.008
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发表时间：
2004-07-01
影响因子：
--
通讯作者：
Yokochi, T
中科院分区：
文献类型：
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作者：
Morikawa, A;Koide, N;Yokochi, T
检查了 D-半乳糖胺 (D-GalN) 对脂多糖 (LPS) 刺激的 RAW 264.7 巨噬细胞中一氧化氮 (NO) 产生的影响。 D-GalN 增加了 LPS 刺激的 RAW 264.7 细胞中 NO 的产生，但不增加肿瘤坏死因子 (TNF)-α。 D-GalN 的预处理增加了 NO 的产生，而其后处理却没有。 D-GalN 增加了用 TNF-α 和干扰素-γ 刺激的 RAW 264.7 细胞中 NO 的产生。 D-GalN 增强 LPS 诱导的 NO 产生是由于诱导型 NO 合酶 mRNA 和蛋白质表达的增强。细胞内活性氧 (ROS) 仅在用 D-GalN 和 LPS 刺激的 RAW 264.7 细胞中产生。细胞内 ROS 的清除消除了 NO 产生的增加。因此，有人认为 D-GalN 可能通过产生细胞内 ROS 来增强 LPS 诱导的 NO 产生。 (C) 2004 年欧洲微生物学会联合会。由 Elsevier B.V. 出版。保留所有权利。
The effect Of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-alpha in LPS-stimulated RAW 264.7 cells. Pretreatment Of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-alpha and interferon-gamma. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.