Drosophila immune priming against Pseudomonas aeruginosa is short-lasting and depends on cellular and humoral immunity.
Drosophila immune priming against Pseudomonas aeruginosa is short-lasting and depends on cellular and humoral immunity.
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DOI：
10.12688/f1000research.2-76.v1
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Apidianakis Y
中科院分区：
文献类型：
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作者：
Christofi T;Apidianakis Y
传统上，免疫反应分为先天臂和适应性臂，两者都存在于脊椎动物中，而只有先天臂存在于无脊椎动物中。在过去的十年里，在黑腹果蝇和其他无脊椎动物身上进行的免疫启动实验挑战了这一教条，质疑了先天免疫和适应性免疫之间的界限。对果蝇反复接种微生物的研究揭示了对特定微生物的长期细胞免疫适应。在这里，我们研究了免疫启动对铜绿假单胞菌感染的持久影响，铜绿假单胞菌是一种对普通果蝇致命的机会性人类病原体。用热致死或低毒力的铜绿假单胞菌启动果蝇，延长苍蝇在与同一物种的毒力菌株的二次致命感染期间的生存。在暴露于持续的低毒力活感染后，保护性免疫反应可以持续10天以上，但在宿主被热杀细菌启动后7天就会消除。此外，不仅是细胞，而且全身NF-κ b介导的免疫反应也有助于免疫启动。因此，每种微生物可能会引发不同的免疫启动机制，这种机制可能会持续很长时间，也可能不会持续很长时间。
Immune responses are traditionally divided into the innate and the adaptive arm, both of which are present in vertebrates, while only the innate arm is found in invertebrates. Immune priming experiments in Drosophila melanogaster and other invertebrates during the last decade have challenged this dogma, questioning the boundaries between innate and adaptive immunity. Studies on repeated inoculation of Drosophila with microbes reveal a long-lasting cellular immunity adaptation against particular microorganisms. Here we study the lasting effect of immune priming against infection with Pseudomonas aeruginosa, an opportunistic human pathogen that is lethal to the common fruit fly. Drosophila priming with heat-killed or low in virulence P. aeruginosa extends fly survival during a secondary lethal infection with a virulent strain of the same species. The protective immune response can last for more than 10 days after exposure to a persistent low-in-virulence live infection, but it is eliminated 7 days after the host is primed with heat-killed bacteria. Moreover, not only the cellular, but also the systemic NF-κB-mediated immune responses contribute to immune priming. Thus each microbe might elicit different mechanisms of immune priming that may or may not last for long.