Fabrication of patterned micromuscles with high activity for powering biohybrid microdevices
Fabrication of patterned micromuscles with high activity for powering biohybrid microdevices
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DOI：
10.1016/j.snb.2005.11.051
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发表时间：
2006-10-12
影响因子：
8.4
通讯作者：
Kim, Byungkyu
中科院分区：
文献类型：
--
作者：
Kim, Deok-Ho;Park, Jungyul;Kim, Byungkyu
在本文中，我们报告了一种微加工方法，用于肌肉细胞的三维图案化生长，为生物混合微设备提供动力。由抗粘附聚乙二醇 (PEG) 微结构限制的微孔在 SiO2 基基底上形成图案，具有可控的表面形貌，用于肌肉细胞培养。使用不同地形高度的 PEG 屏障分析微孔内新生大鼠心肌细胞的形态和运动。研究发现，孤立的心肌聚集体根据细胞的形态和数量表现出各种跳动活动。此外，由 PEG 微结构的更高物理屏障介导的三维生长的心肌细胞比附着在培养皿上胶原蛋白包被表面的细胞（对照）产生更高的收缩力和更快的跳动频率，这表明控制细胞生长的微孔表面形貌可能在工程细胞马达中有用。因此，这里提出的技术将为优化图案化肌肉细胞在构建集成生物驱动微器件中的活动和功能提供一个有价值的平台。 (c) 2005 Elsevier B.V. 保留所有权利。
In this paper, we report a microfabrication approach to patterned three-dimensional growth of muscle cells for powering biohybrid microdevices. The microwells confined by adhesion-resistant polyethylene glycol (PEG) microstructures were patterned on SiO2-based substrate with controllable surface topography for muscle cell culturing. The morphology and motility of neonatal rat cardiac myocytes patterned within microwells were analyzed with different topographical heights of the PEG barrier. It was found that isolated aggregates of cardiac muscles showed various beating activities depending on the morphology and the number of cells. Furthermore, three-dimensionally grown cardiac muscle cells mediated by a higher physical barrier of PEG microstructures generated higher contraction force with faster beating frequency than those of cells attached to the collagen-coated surface on the culture dish (control), suggesting that control over surface topography of microwells for cell growth would be potentially useful in engineering cell motors. Thus the technique presented here would provide a valuable platform for optimizing the activity and functions of patterned muscle cells in building up integrated bioactuated microdevices. (c) 2005 Elsevier B.V. All rights reserved.