Effects of instant coffee consumption on oxidative DNA damage, DNA repair, and redox system in mouse liver.
Effects of instant coffee consumption on oxidative DNA damage, DNA repair, and redox system in mouse liver.
复制标题
饮用速溶咖啡对小鼠肝脏氧化 DNA 损伤、DNA 修复和氧化还原系统的影响。
DOI：
10.1111/j.1750-3841.2009.01192.x
复制
发表时间：
2009
影响因子：
3.9
通讯作者：
T. Hirano
中科院分区：
文献类型：
--
作者：
H. Morii;A. Kuboyama;T. Nakashima;K. Kawai;H. Kasai;K. Tamae;T. Hirano
为了研究速溶咖啡消费对癌症风险的影响，我们分析了咖啡喂养小鼠肝脏中的氧化DNA损伤水平以及DNA修复和氧化还原系统。用/不用0.1%（w/v）速溶咖啡溶液喂养三周龄雄性ICR小鼠。在2、4和8个月时，分析了8-羟基脱氧鸟苷（8-OH-dG）（氧化DNA损伤的主要形式）的水平、小鼠8-OH-dG修复相关基因和氧化还原系统相关基因的表达、SOD活性和LPO水平。同时，一半的小鼠被喂食低维生素（LV）饮食（高压灭菌饮食），以扰乱对氧化应激的防御系统。结果，与0 M对照小鼠和正常饮食（+水）喂养小鼠的肝脏相比，LV饮食（+水）喂养小鼠的肝脏中8-OH-dG水平增加了8个月。然而，在8-OH-dG水平方面，没有观察到饮用水和饮用咖啡之间的显著差异。此外，8-OH-dG修复相关基因的表达，SOD活性和LPO水平也没有显着差异，在所有小鼠组之间的饮水和咖啡饮用。另一方面，在氧化还原系统相关基因中，只有GPx 1的表达发生了变化。这些结果表明，速溶咖啡的消费对氧化应激引起的肝癌风险几乎没有影响。
To examine the effects of instant coffee consumption on cancer risk, we analyzed the oxidative DNA damage levels and the DNA repair and redox systems in the livers of coffee-fed mice. Three-week-old male ICR mice were fed with/without 0.1% (w/v) instant coffee solution. At 2, 4, and 8 mo, the levels of 8-hydroxydeoxyguanosine (8-OH-dG), a major form of oxidative DNA damage, and the expression of mouse 8-OH-dG repair-associated genes and redox system-associated genes, the SOD activity, and the LPO level were analyzed. Simultaneously, half of the mice were fed a low vitamin (LV) diet (autoclaved diet) to disturb the defense system against oxidative stresses. As a result, the 8-OH-dG level was increased in the livers of LV diet (+ water)-fed mice for 8 mo, in comparison to those of the 0 M control mice and normal diet (+ water)-fed mice. However, no significant differences between water drinking and coffee drinking were observed, in terms of the 8-OH-dG level. In addition, the 8-OH-dG repair-associated gene expression, the SOD activity, and the LPO level also showed no significant differences between water drinking and coffee drinking in all mouse groups. On the other hand, among the redox system-associated genes, only the expression of GPx1 was changed. These results suggest that instant coffee consumption has little, if any, effect on the risk of liver cancer due to oxidative stresses.
登录
查看更多内容
影响因子：
7.4
作者：
Hansen, JM;Zhang, H;Jones, DP
通讯作者：
Jones, DP
影响因子：
4.2
作者：
Svilaas, A;Sakhi, AK;Blomhoff, R
通讯作者：
Blomhoff, R
DOI：
10.1073/pnas.94.15.8010
发表时间：
1997-07-22
影响因子：
11.1
作者：
Radicella, JP;Dherin, C;Boiteux, S
通讯作者：
Boiteux, S
影响因子：
2.9
作者：
WOOD, ML;DIZDAROGLU, M;ESSIGMANN, JM
通讯作者：
ESSIGMANN, JM
DOI：
10.1073/pnas.94.14.7429
发表时间：
1997-07-08
影响因子：
11.1
作者：
Rosenquist, TA;Zharkov, DO;Grollman, AP
通讯作者：
Grollman, AP