NIR‐II J‐Aggregated Pt(II)‐Porphyrin‐Based Phosphorescent Probe for Tumor‐Hypoxia Imaging
NIR‐II J‐Aggregated Pt(II)‐Porphyrin‐Based Phosphorescent Probe for Tumor‐Hypoxia Imaging
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NIR – II J – 聚合 Pt(II) – 卟啉 – 用于肿瘤的磷光探针 – 缺氧成像
DOI：
10.1002/adhm.202200467
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发表时间：
2022-05
影响因子：
10
通讯作者：
Wai-Yeung Wong
中科院分区：
文献类型：
--
作者：
Wansu Zhang;Shangyu Chen;Pengfei Sun;Shuai Ye;Quli Fan;Jun Song;Pengju Zeng;Junle Qu;Wai-Yeung Wong
传统的基于磷光的缺氧探头的发光范围仅限于可见光和第一近红外波段(<；1000 nm)，这与光学生物成像的第二近红外波长窗口(NIR-II，1000-1700 nm)相比，具有光散射度高、穿透深度低的缺点。本文报道了5，15-双(2，6-二(十二烷氧基)苯基)卟啉铂(Ⅱ)的J-聚集诱导近红外发光。结晶状态下的X-射线衍射数据证实了聚集体为J-聚集体。此外，固体中PpyPT的发射光谱和激发光谱揭示了J型聚集体中PpyPT的近红外-II发光特征。更重要的是，通过制备具有J-聚集体的水溶性PpyPT纳米粒子(PpyPtNPs4.76)，它具有微秒级的近红外发光寿命和良好的水中氧敏感性。此外，Ppypt NPs4.76在细胞和4T1荷瘤小鼠中表现出良好的生物低氧感知能力。本研究提供了一种通过构建J-聚集体来设计用于灵敏的肿瘤缺氧检测的NIR-II磷光探针的有效策略。
The luminescence of traditional phosphorescence-based hypoxia probes is limited to the visible and first near-infrared wavelength regions (<1000 nm), which has defects of higher light scattering and lower penetration depth in contrast with the second near-infrared wavelength window (NIR-II, 1000-1700 nm) for optical bioimaging. Herein, 5,15-bis(2,6-bis(dodecyloxy)phenyl)-porphyrin platinum(II) (PpyPt) with J-aggregation induced NIR-II phosphorescence is reported. J-aggregates of PpyPt are confirmed by the X-ray diffraction data in the crystalline state. Moreover, the emission and excitation spectra of PpyPt in the solid states reveal NIR-II luminescence feature of PpyPt in J-aggregates. More importantly, by preparation of water-soluble PpyPt nanoparticles (PpyPt NPs4.76 ) with J-aggregates, it has NIR-II phosphorescent lifetime of microseconds and good oxygen-sensitivity in water. Moreover, the good biological hypoxia-sensing potential of PpyPt NPs4.76 is demonstrated in cells and 4T1-tumor-bearing mice. This study provides an efficient strategy to design NIR-II phosphorescent probe for sensitive tumor-hypoxia detection through the construction of J-aggregates.