Release of prostaglandins evoked by neurohormonal stimuli in the isolated spleen of rabbit.
Release of prostaglandins evoked by neurohormonal stimuli in the isolated spleen of rabbit.
复制标题
兔离体脾脏中神经激素刺激引起的前列腺素释放。
DOI：
10.1016/0014-2999(80)90485-9
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发表时间：
1980
影响因子：
5
通讯作者：
K. Malik
中科院分区：
文献类型：
--
作者：
T. Hidaka;K. Malik
在用 Tyrode 溶液灌注的家兔离体脾脏中，刺激交感神经 (NS) 并给予 50-75 ng 去甲肾上腺素 (NA) 或 50 ng 缓激肽 (BK)，将一种物质释放到静脉流出物中，导致大鼠胃条 (RSS)、大鼠结肠 (RC)、鸡直肠 (CR) 和牛冠状动脉 (BCA) 收缩。当将其放置在 37°C 或室温下时，从脾脏中释放的物质的肌肉活性并未减弱。在输注前列腺素 (PG) 合成抑制剂吲哚美辛 (1 μg/ml) 期间，神经激素刺激引起的这种促肌肉物质的释放被消除。将真正的 PGE2 直接应用于测定器官会引起收缩，而 PGI2 会收缩 RSS、RC 和 CR，但会松弛 BCA。在用[14C]AA、NS、NA或BK标记的脾脏中释放具有PGE2以及较小程度的PGF2α的色谱特征的放射性标记产物。吲哚美辛消除了这些放射性标记产物从脾脏的释放。这些观察结果与静脉流出物中物质的肌肉生长特性相结合，表明 PGE2 是由 NS、NA 和 BK 从隔离灌注的兔脾中释放的环加氧酶的主要产物。
In the isolated spleen of rabbit perfused with Tyrode solution, sympathetic nerve stimulation (NS) and administration of either noradrenaline (NA), 50–75 ng, or bradykinin (BK), 50 ng, released a material into the venous effluent that cause contraction of rat stomach strip (RSS), rat colon (RC), chick rectum (CR) and bovine coronary artery (BCA). The musculotropic activity of the material released from the spleen was not diminished when it was allowed to stand at either 37°C or at room temperature. The release of this musculotropic material evoked by neurohormonal stimuli was abolished during the infusion of the prostaglandin (PG) synthesis inhibitor, indomethacin, 1 μg/ml. Application of authentic PGE2directly on the assay organs caused contraction, whereas PGI2contracted RSS, RC, and CR but relaxed BCA. In the spleen labeled with [14C]AA , NS, NA or BK released radio-labeled products haveing the chromatographic characteristics of PGE2and, to a lesser extent, of PGF2α. Indomethacin abolished the release of these radiolabeled products from the spleen. These observations taken together with the musculotropic characteristics of the material in the venous effluent, suggest that PGE2is the major product of cyclo-oxygenase released by NS, NA and by BK from the isolation perfused spleen of rabbit.