Altered expression of signalling lymphocyte activation molecule (SLAM) family receptors CS1 (CD319) and 2B4 (CD244) in patients with systemic lupus erythematosus
Altered expression of signalling lymphocyte activation molecule (SLAM) family receptors CS1 (CD319) and 2B4 (CD244) in patients with systemic lupus erythematosus
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DOI：
10.1111/j.1365-2249.2010.04116.x
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发表时间：
2010-06-01
影响因子：
4.6
通讯作者：
Mathew, P. A.
中科院分区：
文献类型：
--
作者：
Kim, J. R.;Mathew, S. O.;Mathew, P. A.
P>CS1 （CRACC, CD319）和2B4 （CD244）是信号淋巴细胞激活分子（SLAM）家族受体的成员，调节多种免疫功能。编码SLAM家族受体的基因位于1q23，与系统性红斑狼疮（SLE）有关。在这项研究中，我们研究了CS1和2B4在SLE患者免疫细胞中的表达和选择性剪接。用流式细胞术分析了45例SLE患者和30例健康人外周血总单核细胞（PBMCs）、T、B、NK细胞和单核细胞表面CS1和2B4的表达。SLE患者cs1阳性B细胞群显著增加。由于CS1是一种自配体，CS1的亲同性相互作用诱导B细胞增殖和自分泌细胞因子分泌，这可能是SLE中自身反应性B细胞增殖的原因。与健康对照组相比，SLE患者NK细胞和单核细胞表面表达2B4的比例显著降低。我们的研究证实了CS1和2B4剪接变体在SLE患者PBMC中介导差异信号的表达改变。
P>CS1 (CRACC, CD319) and 2B4 (CD244), members of the signalling lymphocyte activation molecule (SLAM) family receptors, regulate various immune functions. Genes encoding SLAM family receptors are located at 1q23, implicated in systemic lupus erythematosus (SLE). In this study, we have investigated the expression and alternative splicing of CS1 and 2B4 in immune cells from SLE patients. The surface expression of CS1 and 2B4 on total peripheral blood mononuclear cells (PBMCs), T, B, natural killer (NK) cells and monocytes in 45 patients with SLE and 30 healthy individuals was analysed by flow cytometry. CS1-positive B cell population was increased significantly in SLE patients. Because CS1 is a self-ligand and homophilic interaction of CS1 induces B cell proliferation and autocrine cytokine secretion, this could account for autoreactive B cell proliferation in SLE. The proportion of NK cells and monocytes expressing 2B4 on their surface was significantly lower in patients with SLE compared to healthy controls. Our study demonstrated altered expression of splice variants of CS1 and 2B4 that mediate differential signalling in PBMC from patients with SLE.