Expression and functional analysis of the plant-specific histone deacetylase HDT701 in rice

Expression and functional analysis of the plant-specific histone deacetylase HDT701 in rice
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植物特异性组蛋白脱乙酰酶HDT701在水稻中的表达及功能分析

DOI:
10.3389/fpls.2014.00764
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发表时间:
2015-01
影响因子:
5.6
通讯作者:
Duan, Jun
Duan, Jun
中科院分区:
生物学2区
文献类型:
--
作者:
Zheng, Feng;Tian, Lining;Liu, Xuncheng;Duan, Jun

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组蛋白尾部N端的可逆乙酰化和去乙酰化在调节真核基因活性中起着至关重要的作用。核心组蛋白的乙酰化与基因激活相关,而组蛋白的去乙酰化通常与基因阻遏相关。组蛋白乙酰化水平由组蛋白乙酰转移酶(HAT)和组蛋白脱乙酰酶(HDAC)拮抗催化。在这项工作中,我们研究了HDT701的亚细胞定位,表达模式和功能,植物特异性HD2型组蛋白去乙酰化酶在水稻中的成员。HDT701定位于细胞核的亚细胞水平。组织化学GUS染色分析表明,HDT701在水稻的整个生命周期中组成型表达。HDT701在水稻中的过表达降低了种子萌发过程中的阿坝、盐和渗透胁迫抗性。HDT701过表达株系种子萌发延迟与组蛋白H4乙酰化降低和GA生物合成基因表达下调有关。此外,HDT701在水稻中的过表达增强了幼苗期的盐和渗透胁迫抗性。综上所述,我们的研究结果表明,HDT701可能在调节水稻种子萌发响应非生物胁迫中发挥重要作用。
Reversible histone acetylation and deacetylation at the N-terminus of histone tails play a crucial role in regulating eukaryotic gene activity. Acetylation of core histones is associated with gene activation, whereas deacetylation of histone is often correlated with gene repression. The level of histone acetylation is antagonistically catalyzed by histone acetyltransferases citation(HATs) and histone deacetylases (HDACs). In this work, we examined the subcellular localization, expression pattern and function of HDT701, a member of the plant-specific HD2-type histone deacetylase in rice. HDT701 is localized at the subcellular level in the nucleus. Histochemical GUS-staining analysis revealed that HDT701 is constitutively expressed throughout the life cycle of rice. Overexpression of HDT701 in rice decreases ABA, salt and osmotic stress resistance during seed germination. Delayed seed germination of HDT701 overexpression lines is associated with decreased histone H4 acetylation and down-regulated expression of GA biosynthetic genes. Moreover, overexpression of HDT701 in rice enhances salt and osmotic stress resistance during the seedling stage. Taken together, our findings suggested that HDT701 may play an important role in regulating seed germination in response to abiotic stresses in rice.
DOI: 10.1021/bi961294x
发表时间: 1996-12-10
期刊: BIOCHEMISTRY
影响因子: 2.9
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期刊: The EMBO Journal
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发表时间: 2007-02-23
期刊: CELL
影响因子: 64.5
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