Is Drosophila-microbe association species-specific or region specific? A study undertaken involving six Indian Drosophila species
Is Drosophila-microbe association species-specific or region specific? A study undertaken involving six Indian Drosophila species
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DOI：
10.1007/s11274-017-2261-2
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发表时间：
2017-05
影响因子：
4.1
通讯作者：
Kopal Singhal;Radhika Khanna;S. Mohanty
中科院分区：
文献类型：
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作者：
Kopal Singhal;Radhika Khanna;S. Mohanty
目前的工作旨在利用下一代测序（NGS）技术识别与六种印度果蝇物种相关的微生物多样性，并发现它们在物种和生态地理区域中分布的性质。六个果蝇物种的全果蝇 gDNA 使用 NGS 技术在 Illumina 平台中生成序列。使用 BLASTn 对基于 NCBI 的 NR 数据库的从头组装的原始读数进行爆破，以鉴定其细菌负荷。我们试图纳入来自不同分类群和亚群以及来自印度三个不同生态气候地区的果蝇物种；四种属于印度中部，其余两种，D.黑腹果蝇和D. ananassae 属于印度西部和南部，以确定其物种和区域分布。我们在所有 6 个研究物种中检测到 33 个细菌属的存在，其中以变形菌纲为主。其中，D.黑腹果蝇被发现是最具多样性的，携带约 85% 的细菌多样性。我们的研究结果推断出果蝇宿主中细菌物种的物种特异性和环境特异性。尽管目前的结果与大多数早期研究一致，但也与一些研究不一致。目前关于宿主-细菌关联及其物种特异性适应的研究结果可能为理解宿主-微生物相互作用以及微生物对宿主生理学的表型影响提供一些见解。所获得的知识可能会重要地应用于最近将在印度和国外通过肠道微生物群落实施的昆虫和害虫种群控制策略。
The present work aims to identify the microbial diversity associated with six IndianDrosophilaspecies using next generation sequencing (NGS) technology and to discover the nature of their distribution across species and eco-geographic regions. Whole fly gDNA of sixDrosophilaspecies were used to generate sequences in an Illumina platform using NGS technology. De novo based assembled raw reads were blasted against the NR database of NCBI using BLASTn for identification of their bacterial loads. We have tried to includeDrosophilaspecies from different taxonomical groups and subgroups and from three different eco-climatic regions India; four species belong to Central India, while the rest two,D. melanogasterandD. ananassae, belong to West and South India to determine both their species-wise and region-wide distribution. We detected the presence of 33 bacterial genera across all six study species, predominated by the class Proteobacteria. Amongst all,D. melanogasterwas found to be the most diverse by carrying around 85% of the bacterial diversity. Our findings infer both species-specific and environment-specific nature of the bacterial species inhabiting theDrosophilahost. Though the present results are consistent with most of the earlier studies, they also remain incoherent with some. The present study outcome on the host-bacteria association and their species specific adaptation may provide some insight to understand the host-microbial interactions and the phenotypic implications of microbes on the host physiology. The knowledge gained may be importantly applied into the recent insect and pest population control strategy going to implement through gut microflora in India and abroad.