Structure and expression of the herpes simplex virus type 2 glycoprotein gB gene
Structure and expression of the herpes simplex virus type 2 glycoprotein gB gene
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单纯疱疹病毒2型糖蛋白gB基因的结构和表达
DOI：
10.1128/jvi.61.2.326-335.1987
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发表时间：
1987
影响因子：
5.4
通讯作者：
R. Burke
中科院分区：
文献类型：
--
作者：
L. Stuve;S. Brown‐Shimer;C. Pachl;R. Najarian;D. Dina;R. Burke
单纯疱疹病毒 2 型 333 株的糖蛋白 gB2 基因已被克隆、测序并在哺乳动物细胞中表达。 gB2蛋白与同源gB1蛋白的总体核苷酸和氨基酸序列同源性为86%。然而，在 125 个氨基酸取代或缺失中，只有 12.5% 是保守取代。这些差异集中在 NH2 末端区域、中心区域和 COOH 末端区域内，导致接近同一性的域被明显差异的小区域打破。最大同源性区域包括从残基 484 开始的 90 个氨基酸序列和跨越残基 835 至 873 的 39 个氨基酸，它们分别覆盖了 Bzik 等人在 gB1 中映射到 Ala-552 的进入速率基因座和映射到 Arg-857 的同步基因座。 （D. J. Bzik、B. A. Fox、N. A. DeLuca 和 S. Person，病毒学 133：301-314，1984）。佩莱特等人。 (P.E.Pellett，K.G.Kousoulas，L.Pereira，和 B.Roizman，J.Virol.53：243-253，1985)将三个单克隆抗体抗性 gB1 突变体中的突变定位在氨基酸 273 和 443 之间。这些表位包含在 gB1 和 gB2 之间具有 98 个相同残基的区域中。通过将缺少 gB2 的 303 个羧基末端氨基酸的截短基因放入哺乳动物 COS 和 CHO 细胞中，验证了该蛋白质的身份。通过免疫荧光和放射免疫沉淀证实表达。该蛋白将从稳定的 CHO 细胞系中纯化出来，并与 gB1 在动物攻击模型中的免疫原性和保护功效进行比较。
The gene for glycoprotein gB2 of herpes simplex virus type 2 strain 333 was cloned, sequenced, and expressed in mammalian cells. The gB2 protein had an overall nucleotide and amino acid sequence homology of 86% with the cognate gB1 protein. However, of the 125 amino acid substitutions or deletions, only 12.5% were conservative replacements. These differences were clustered within an NH2-terminal region, a central region, and a COOH-terminal region, resulting in domains of near identity broken by small regions of marked divergence. Regions of greatest homology included a 90-amino-acid stretch starting at residue 484 and 39 amino acids spanning residues 835 to 873, which cover a rate-of-entry locus mapped to Ala-552 and a syn locus mapped to Arg-857, respectively, in gB1 by Bzik et al. (D. J. Bzik, B. A. Fox, N. A. DeLuca, and S. Person, Virology 133:301-314, 1984). Pellett et al. (P. E. Pellett, K. G. Kousoulas, L. Pereira, and B. Roizman, J. Virol. 53:243-253, 1985) mapped the mutations in three monoclonal antibody-resistant gB1 mutants between amino acids 273 and 443. These epitopes are included in a region of 98 residues identical between gB1 and gB2. The identity of this protein was verified by placing a truncated gene lacking the 303 carboxyl-terminal amino acids of gB2 into mammalian COS and CHO cells. Expression was demonstrated by immunofluorescence and radioimmunoprecipitation. This protein will be purified from the stable CHO cell lines and compared with gB1 for immunogenicity and protective efficacy in animal challenge models.
单纯疱疹病毒 DNA 克隆片段的异源双链分析。
影响因子：
3.7
作者：
Kudler,L;Jones,TR;Russell,RJ;Hyman,RW
通讯作者：
Hyman,RW
DOI：
10.1073/pnas.77.7.4216
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
URLAUB, G;CHASIN, LA
通讯作者：
CHASIN, LA