Hypoxia responsive gene expression is mediated by various subsets of transcription factors and miRNAs that are determined by the actual oxygen availability
Hypoxia responsive gene expression is mediated by various subsets of transcription factors and miRNAs that are determined by the actual oxygen availability
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DOI：
10.1111/j.1469-8137.2010.03451.x
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发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
van Dongen, Joost T.
中科院分区：
文献类型：
--
作者：
Licausi, Francesco;Weits, Daan A.;van Dongen, Joost T.
氧可用性降低不仅与洪水有关，而且在生长和发育过程中也会发生。我们采用定量PCR方法分析了不同低氧条件下拟南芥根中1900多种转录因子（TF）和180种microRNA初级转录本（pri-miRNAs）的表达。我们还分析了由缺氧诱导的基因的启动子相对于过度代表的DNA元素，可以作为潜在的TF结合位点和它们在体内的相互作用进行了验证。我们确定了不同的TF子集，通过时间和氧浓度依赖性的方式响应差异。验证了所选TF及其预测的DNA结合元件的调节潜力。虽然pri-miRNAs的表达在缺氧条件下受到不同的调节，但只有一种相应的成熟miRNAs发生相应的变化。我们的研究结果表明，缺氧诱导基因的调控是通过多种TF组合的同时相互作用来控制的。在缺氧条件下，诱导了一组额外的TF。通过miRNA调控基因表达似乎在缺氧过程中起次要作用。
P>Reduced oxygen availability is not only associated with flooding, but occurs also during growth and development. It is largely unknown how hypoxia is perceived and what signaling cascade is involved in activating adaptive responses.We analysed the expression of over 1900 transcription factors (TFs) and 180 microRNA primary transcripts (pri-miRNAs) in Arabidopsis roots exposed to different hypoxic conditions by means of quantitative PCR. We also analysed the promoters of genes induced by hypoxia with respect to over-represented DNA elements that can act as potential TF binding sites and their in vivo interaction was verified.We identified various subsets of TFs that responded differentially through time and in an oxygen concentration-dependent manner. The regulatory potential of selected TFs and their predicted DNA binding elements was validated. Although the expression of pri-miRNAs was differentially regulated under hypoxia, only one corresponding mature miRNA changed accordingly. Putative target transcripts of the miRNAs were not significantly affected.Our results show that the regulation of hypoxia-induced genes is controlled via simultaneous interaction of various combinations of TFs. Under anoxic conditions, an additional set of TFs is induced. Regulation of gene expression via miRNAs appears to play a minor role during hypoxia.