The C-terminus of IFN gamma is sufficient for intracellular function.
The C-terminus of IFN gamma is sufficient for intracellular function.
复制标题
IFN γ 的 C 末端足以维持细胞内功能。
DOI：
10.1006/bbrc.1994.2375
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发表时间：
1994
影响因子：
3.1
通讯作者：
Johnson,HW
中科院分区：
文献类型：
--
作者：
Szente,BE;Soos,JM;Johnson,HW
We have previously shown that murine interferon gamma (IFNγ) and its C-terminal peptide, muIFNγ(95-133), bind to a region on the cytoplasmic domain of the IFNγ receptor contained in the synthetic peptide, MIR(253-287). This region of the murine receptor bears considerable homology (≍ 80%) to its human counterpart. Here we report that not only do human IFNγ and the human IFNγ C-terminal peptide, huIFNγ(95-134), bind to the cytoplasmic domain of the human IFNγ receptor, but also that this interaction is species non-specific. MuIFNγ(95-133) binds to human IFNγ receptor cytoplasmic peptide HIR(252-291), and huIFNγ(95-134) binds to MIR(253-287). Furthermore, treatment of murine macrophage cell lines with C-terminal peptides of either murine or human IFNγ results in 10-fold upregulation of MHC class II molecule expression and increased resistance to infection with vesicular stomatitis virus (VSV) (106- 109-fold reduction in yield). These data suggest a direct role for the C-terminus of IFNγ in the initiation of intracellular signalling processes and may be indicative of a more general mechanism of action for extracellular signalling molecules.
