CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus.
CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus.
复制标题
CD4 是人类疱疹病毒 7 型受体的重要组成部分：干扰人类免疫缺陷病毒。
DOI：
10.1073/pnas.91.9.3872
复制
发表时间：
1994
影响因子：
11.1
通讯作者：
R. Gallo
中科院分区：
文献类型：
--
作者：
P. Lusso;P. Secchiero;R. Crowley;A. Garzino;Z. Berneman;R. Gallo
在这项研究中，我们证明，糖蛋白CD 4，免疫球蛋白超家族的成员，是人类疱疹病毒7型（HHV-7），最近发现的T淋巴细胞的人类疱疹病毒的受体的关键组成部分。在HHV-7感染过程中，观察到人CD 4 + T细胞表面膜表达的选择性和进行性下调。各种鼠抗CD 4单克隆抗体和重组可溶形式的人CD 4引起剂量依赖性抑制HHV-7感染的主要CD 4 + T淋巴细胞。此外，放射性标记的HHV-7特异性结合表达人CD 4的宫颈癌细胞（HeLa）。标记的癌细胞（HeLa）表达人CD 4。在HHV-7和人类免疫缺陷病毒（HIV）之间观察到明显的相互干扰，HIV是导致获得性免疫缺陷综合征的逆转录病毒，也使用CD 4作为受体。先前暴露于HHV-7的CD 4 + T细胞显著干扰了原代和体外传代的HIV-1分离株的感染。反过来，持续感染HIV-1或用可溶性形式的gp 120（HIV-1的CD 4结合包膜糖蛋白）治疗，使CD 4 + T细胞对HHV-7感染具有抗性。这些数据表明，CD 4在HHV-7的受体机制中起关键作用。HHV-7和HIV之间的拮抗作用可用于设计艾滋病的治疗方法。
In this study, we demonstrate that the glycoprotein CD4, a member of the immunoglobulin superfamily, is a critical component of the receptor for human herpesvirus 7 (HHV-7), a recently discovered T-lymphotropic human herpesvirus. A selective and progressive downregulation of the surface membrane expression of CD4 was observed in human CD4+ T cells in the course of HHV-7 infection. Various murine monoclonal antibodies to CD4 and the recombinant soluble form of human CD4 caused a dose-dependent inhibition of HHV-7 infection in primary CD4+ T lymphocytes. Moreover, radiolabeled HHV-7 specifically bound to cervical carcinoma cells (HeLa) expressing human CD4. A marked carcinoma cells (HeLa) expressing human CD4. A marked reciprocal interference was observed between HHV-7 and human immunodeficiency virus (HIV), the retrovirus that causes the acquired immunodeficiency syndrome and also uses CD4 as a receptor. Previous exposure of CD4+ T cells to HHV-7 dramatically interfered with infection by both primary and in vitro-passaged HIV-1 isolates. Reciprocally, persistent infection with HIV-1 or treatment with the soluble form of gp120, the CD4-binding envelope glycoprotein of HIV-1, rendered CD4+ T cells resistant to HHV-7 infection. These data indicate that CD4 is critically involved in the receptor mechanism for HHV-7. The antagonistic effect between HHV-7 and HIV could be exploited to devise therapeutic approaches to AIDS.