Centrobin controls mother-daughter centriole asymmetry in Drosophila neuroblasts
Centrobin controls mother-daughter centriole asymmetry in Drosophila neuroblasts
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DOI：
10.1038/ncb2671
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发表时间：
2013-03-01
影响因子：
21.3
通讯作者：
Gonzalez, C.
中科院分区：
文献类型：
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作者：
Januschke, J.;Reina, J.;Gonzalez, C.
在果蝇神经母细胞的间期期间，中心蛋白（CNB）阳性的子体中心粒保留了中心粒周围物质（PCM）并组织了紫苑，紫苑是细胞分裂方向的关键决定因素。在这里，我们表明，缺乏 CNB 的子代中心粒无法实现这一功能，而携带异位 CNB 的母体中心粒可以。 CNB 与一组中心体蛋白共沉淀，包括 gamma-TUB、ANA2、CNN、SAS-4、ASL、DGRIP71、POLO 和 SAS-6。对 POLO 进行化学抑制或去除 CNB 中存在的三个 POLO 磷酸化位点后，间期微管 aster 就会丢失。这些结果表明，中心粒 CNB 定位对于使中心粒保留 PCM 并组织果蝇神经母细胞中的间期紫苑是必要且充分的。他们还揭示了 POLO 在此过程中的间期功能，该功能似乎选择了参与有丝分裂中心体成熟的蛋白质网络的一部分。
During interphase in Drosophila neuroblasts, the Centrobin (CNB)-positive daughter centriole retains pericentriolar material (PCM) and organizes an aster that is a key determinant of the orientation of cell division. Here we show that daughter centrioles depleted of CNB cannot fulfil this function whereas mother centrioles that carry ectopic CNB can. CNB co-precipitates with a set of centrosomal proteins that include gamma-TUB, ANA2, CNN, SAS-4, ASL, DGRIP71, POLO and SAS-6. Following chemical inhibition of POLO or removal of three POLO phosphorylation sites present in CNB, the interphase microtubule aster is lost. These results demonstrate that centriolar CNB localization is both necessary and sufficient to enable centrioles to retain PCM and organize the interphase aster in Drosophila neuroblasts. They also reveal an interphase function for POLO in this process that seems to have co-opted part of the protein network involved in mitotic centrosome maturation.