In vivo function of a novel Siah protein in Drosophila
In vivo function of a novel Siah protein in Drosophila
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DOI：
10.1016/j.mod.2007.04.007
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发表时间：
2007-08-01
影响因子：
2.6
通讯作者：
Cooper, Sarah E.
中科院分区：
文献类型：
--
作者：
Cooper, Sarah E.
Siah蛋白是果蝇Sina蛋白的哺乳动物同源物，其功能是E3泛素连接酶，并针对多种细胞蛋白进行降解。在这里，我研究了果蝇蛋白Sina- homologue （SinaH）的体内功能，它与Sina高度相似。完全缺乏SinaH的果蝇可以存活，并且与基因突变Ebi结合，显示出额外的背中央刚毛表型。我还表明SinaH和Ebi在体内和体外都可以相互作用，这表明它们在相同的物理复合物中起作用。缺乏新浪和新浪同源基因的果蝇也被创造出来，并显示出可见的眼睛和鬃毛表型，这可以用无法降解神经元抑制因子Tramtrack来解释。我没有发现新浪和新浪功能冗余的证据。2007爱思唯尔爱尔兰有限公司版权所有。
The Siah proteins, mammalian homologues of the Drosophila Sina protein, function as E3 ubiquitin ligase enzymes and target a wide range of cellular proteins for degradation. Here, I investigate the in vivo function of the fly protein, Sina-Homologue (SinaH), which is highly similar to Sina. Flies that completely lack SinaH are viable and in combination with a mutation in the gene, Ebi, show an extra dorsal central bristle phenotype. I also show that SinaH and Ebi can interact with each other both in vivo and in vitro suggesting that they act in the same physical complex. Flies that lack both Sina and Sina-Homologue were also created and show visible eye and bristle phenotypes, which can be explained by an inability to degrade the neuronal repressor, Tramtrack. I find no evidence for redundancy in the function of Sina and SinaH. (c) 2007 Elsevier Ireland Ltd. All rights reserved.