DLA-D-specific suppressor cells characterized by monoclonal antibodies.
DLA-D-specific suppressor cells characterized by monoclonal antibodies.
复制标题
以单克隆抗体为特征的 DLA-D 特异性抑制细胞。
DOI：
10.1097/00007890-198502000-00015
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发表时间：
1985
期刊：
影响因子：
6.2
通讯作者：
Storb,R
中科院分区：
文献类型：
--
作者：
Szer,J;Deeg,HJ;Severns,E;Storb,R
犬淋巴细胞同种抗原引发的混合白细胞培养（MLC）的能力，以抑制MLC的反应进行了测试。将这些细胞以1：50和1：20的比例添加到MLC中的新鲜应答细胞中，并且抑制反应性分别为90 $$7%（平均$$ SD）和91 $$3%。在较低的比率下，未观察到抑制。单克隆抗体E11（IgG 3）识别犬Thy 1+ T细胞的一个亚群，并与补体治疗能够消耗抑制细胞从散装MLC（11 $$6%的控制抑制），但DT-2（IgG 2a），另一种犬T细胞抗体，没有这样的效果（84 $$11%）（P= 0.028）。抗体A5（IgG 2b），与所有的T细胞和大多数非T细胞反应，也消除了抑制。阳性选择的E11+ bulk-MLC细胞受到抑制（91%），而E11− bulk-MLC细胞没有受到抑制（4%）。在所描述的比率下，抑制对刺激物细胞的DLA-D表型是特异性的。在使用原始刺激器的测试中，抑制率为91 $$7%;在使用DLA-D相同刺激器的测试中，抑制率为92 $$5%;对于DLA-D不相同刺激器，抑制率为38 $$25%（P= 0.005）。非特异性抑制随着抑制细胞数量的增加而增加。在1：10和1：5的比例下，非特异性抑制分别为72 $$13%和92 $$8%。这些研究表明，在适当的条件下，Thy 1+，E11+，A5+，DT-2-同种抗原激活的犬T淋巴细胞具有DLA-D特异性抑制细胞的功能，而DT-2+细胞则不具有这种功能。
Canine lymphocytes alloantigen-primed in mixed leukocyte culture (MLC) were tested for their ability to suppress MLC reactivity. These cells were added to fresh responder cells in MLC at ratios of 1: 50 and 1: 20 and suppressed reactivity 90$ $7%(mean $$ SD) and 91$ $3% respectively. At lower ratios no suppression was observed. Monoclonal antibody E11 (IgG3) recognized a subset of canine Thy 1+ T cells, and with complement treatment was capable of depleting suppressor cells from bulk MLC (11$ $6% of control suppression), but DT-2 (IgG2a), another canine T-cell antibody, had no such effect (84$ $11%)(P= 0.028). Antibody A5 (IgG2b), reactive with all T and most non-T cells, also eliminated suppression. Positively selected E11+ bulk-MLC cells suppressed (91%) and E11− bulk-MLC cells did not (4%). At the ratios described, suppression was specific for the DLA-D phenotype of the stimulators, cells. In tests with the original stimulators, suppression was 91$ $7%; in tests with DLA-D identical stimulators it was 92$ $5%; and for DLA-D nonidentical stimulators it was 38$ $25%(P= 0.005). Nonspecific suppression increased with increasing numbers of suppressor cells. At ratios of 1: 10 and 1: 5 nonspecific suppression was 72$ $13% and 92$ $8%, respectively. These studies show that under appropriate conditions, Thy1+, E11+, A5+, DT-2-alloantigenactivated canine T lymphocytes function as DLA-Dspecific suppressor cells and DT-2+ cells do not.