Regulation of hyperactivation by PPP2 in hamster spermatozoa.
Regulation of hyperactivation by PPP2 in hamster spermatozoa.
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DOI：
10.1530/rep-08-0366
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发表时间：
2010-05
期刊：
影响因子：
3.8
通讯作者：
Tatsuya Suzuki;M. Fujinoki;H. Shibahara;Mitsuaki Suzuki
中科院分区：
文献类型：
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作者：
Tatsuya Suzuki;M. Fujinoki;H. Shibahara;Mitsuaki Suzuki
目前普遍认为，丝氨酸/苏氨酸蛋白磷酸酶(PPP)与精子过度激活的调节有关。在本研究中，我们研究了与调节仓鼠精子过度激活相关的PPP的类型。蛋白磷酸酶PPP1CA、PPP1CC、PPP2和PPP3存在于仓鼠精子中。在使用几种抑制剂的实验中，至少抑制PPP2会增强精子的过度激活，尽管抑制PPP1也会增强精子的过度激活。有趣的是，在PPP2变成不活跃的形式后，精子被过度激活。然后，PPP1CA在精子被过度激活后成为一种活性形式。酪氨酸磷酸化与精子过度激活的调节密切相关，这一点也已被广泛接受。当PPP2被抑制时，酪氨酸磷酸化完全没有增强。另一方面，抑制PPP1可促进酪氨酸磷酸化。从结果来看，PPP2可能与精子过度激活的调节密切相关，尽管它与酪氨酸磷酸化的调节无关。
It has been widely accepted that serine/threonine protein phosphatases (PPPs) are associated with the regulation of sperm hyperactivation. In the present study, we examined the types of PPPs associated with the regulation of hamster sperm hyperactivation. Protein phosphatases PPP1CA, PPP1CC, PPP2, and PPP3 are present in hamster sperm. In the experiments using several inhibitors, sperm hyperactivation was enhanced when PPP2 was inhibited at least, although inhibition of PPP1 also enhanced sperm hyperactivation. Interestingly, sperm were hyperactivated after PPP2 became an inactive form. And then, PPP1CA became an active form after sperm were hyperactivated. It has also been widely accepted that tyrosine phosphorylation is closely associated with the regulation of sperm hyperactivation. When PPP2 was inhibited, tyrosine phosphorylation was not enhanced at all. On the other hand, inhibition of PPP1 enhanced tyrosine phosphorylation. From the results, it is likely that PPP2 is closely associated with the regulation of sperm hyperactivation, although it is not associated with the regulation of tyrosine phosphorylation.