Molecular cloning and characterization of prokineticin receptors
Molecular cloning and characterization of prokineticin receptors
复制标题
DOI：
10.1016/s0167-4781(02)00546-8
复制
发表时间：
2002-12-12
期刊：
影响因子：
--
通讯作者：
Furuichi, K
中科院分区：
文献类型：
--
作者：
Soga, T;Matsumoto, SI;Furuichi, K
最近的研究发现了两个新的生物功能蛋白，命名为prokineticin 1/EG-VEGF和prokineticin 2，它们是曼巴MITI和青蛙Bv8的哺乳动物同源物。原激动素通过G蛋白偶联受体(GPCRs)发挥生理功能。在这项研究中，我们报告了两个内源性原激动素受体的分子鉴定，命名为PK-R1和PK-R2，通过搜索人类基因组DNA数据库。位于第2染色体的PK-R1和位于染色体20p13的PK-R2具有87%的同源性，在已知的GPCR中具有极高的同源性。在功能分析中，表达PK-Rs的哺乳动物细胞对原激动素的反应是浓度依赖的。组织分布分析显示，PK-R1在睾丸、延髓、骨骼肌和皮肤中均有表达，而PK-R2在中枢神经系统中优先表达。本文报道的PK-Rs的组织分布表明，原激动素在体内发挥着多功能作用。(C)2002 Elsevier Science B.V.保留所有权利。
Recent studies have identified two novel biofunctional proteins, termed prokineticin 1/EG-VEGF and prokineticin 2, which were mammalian homologues of mamba MITI and frog Bv8. Prokineticins have been demonstrated to exert their physiological functions through G-protein coupled receptors (GPCRs). In this study, we report the molecular identification of two endogenous prokineticin receptors, designated PK-R1 and PK-R2, through a search of the human genomic DNA database. PK-R1, locating in chromosome 2, and PK-R2, locating in chromosome 20p13, shared 87% homology, which was an extremely high value among known GPCRs. In functional assays, mammalian cells expressing PK-Rs responded to prokineticins in a concentration-dependent manner. Tissue distribution analysis revealed that expression of PK-R1 was observed in the testis, medulla oblongata, skeletal muscle and skin, while that of PK-R2 showed preferential expression in the central nervous system. The tissue distribution of PK-Rs reported in this paper suggests that the prokineticins play multifunctional roles in vivo. (C) 2002 Elsevier Science B.V. All rights reserved.