Genomic analysis of uncultured marine viral communities
Genomic analysis of uncultured marine viral communities
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DOI：
10.1073/pnas.202488399
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发表时间：
2002-10-29
影响因子：
11.1
通讯作者：
Rohwer, F
中科院分区：
文献类型：
--
作者：
Breitbart, M;Salamon, P;Rohwer, F
病毒是海洋中数量级上最常见的生物实体。然而，人们对它们的多样性知之甚少。在这里，我们报告了两个未培养的海洋病毒群落的基因组分析。超过65%的序列与先前报道的序列不显着相似，这表明许多多样性以前未被表征。在已知的序列中，最常见的重大打击是针对病毒的。被击中的病毒包括来自所有主要的dsDNA尾噬菌体家族的序列，以及一些藻类病毒。几个基于观察到的contigs数量的独立数学模型预测，最丰富的病毒基因组占两个群落总种群的2-3%，估计包含374到7114种病毒类型。总的来说，病毒群落的多样性非常高。结果还表明，对未培养的海洋病毒群落的整个基因组进行测序是可能的。
Viruses are the most common biological entities in the oceans by an order of magnitude. However, very little is known about their diversity. Here we report a genomic analysis of two uncultured marine viral communities. Over 65% of the sequences were not significantly similar to previously reported sequences, suggesting that much of the diversity is previously uncharacterized. The most common significant hits among the known sequences were to viruses. The viral hits included sequences from all of the major families of dsDNA tailed phages, as well as some algal viruses. Several independent mathematical models based on the observed number of contigs predicted that the most abundant viral genome comprised 2-3% of the total population in both communities, which was estimated to contain between 374 and 7,114 viral types. Overall, diversity of the viral communities was extremely high. The results also showed that it would be possible to sequence the entire genome of an uncultured marine viral community.