Modeling Individual Recovery after Peripheral Nerve Injury in Rats and the Effects of Parturition
Modeling Individual Recovery after Peripheral Nerve Injury in Rats and the Effects of Parturition
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DOI：
10.1097/aln.0000000000000360
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发表时间：
2014-11-01
期刊：
影响因子：
8.8
通讯作者：
Eisenach, James C.
中科院分区：
文献类型：
--
作者：
Aschenbrenner, Carol A.;Houle, Timothy T.;Eisenach, James C.
背景：手术后疼痛的恢复表现出很大的个体差异，恢复非常缓慢，相当于慢性疼痛。产后的手术损伤会适度增加主要神经损伤后的初始恢复。在这项研究中，作者使用恢复时间超过 2 至 3 个月的神经损伤，并应用生长曲线模型来进一步了解产后期对恢复速度的影响。 方法：在部分脊神经结扎之前和之后 10 周内，测定了 41 只 Sprague-Dawley 大鼠对后爪机械刺激的退缩阈值。研究人员对年龄匹配的雄性和雌性大鼠以及产后带着幼崽或在分娩时与幼崽分开的雌性大鼠进行了研究。尽管各组的测量时间不同且数据缺失，但仍将生长曲线分析应用于手术后恢复模型，并将这些结果与双向重复测量方差分析的结果进行比较。结果：男性和女性的恢复时间过程相似。相比之下，产后组的恢复速度更快，术后第 6 天至 66 天之间建模轨迹的 95% CI 不重叠。与方差分析相比，生长曲线分析在大多数时间段的 CI 更精确。结论：作者描述了一种量化大鼠手术后超敏反应恢复的分析方法，与传统使用的方法相比，该方法具有几个明显的优势。研究结果并不支持恢复轨迹存在性别差异，但证实并扩展了之前的观察结果，即产科分娩时的损伤与异常快速的恢复有关。
Background: Recovery from pain after surgery exhibits large interindividual variability, with very slow recovery equated to chronic pain. Surgical injury in the postpartum period modestly increases initial recovery after major nerve injury. In this study, the authors use a nerve injury that recovers over 2 to 3 months and apply growth curve modeling to further understand the effect of the postpartum period on speed of recovery.Methods: Withdrawal threshold to mechanical stimulus on the hind paw was determined in 41 Sprague-Dawley rats before and for 10 weeks after partial spinal nerve ligation. Age-matched male and female rats and postpartum females with pups or those separated from pups at delivery were studied. Growth curve analyses were applied to model recovery after surgery despite varying timing of measurements across groups and missing data, and these results were compared with those of two-way repeated-measures ANOVA.Results: The recovery time course was similar between males and females. In contrast, recovery was hastened in the postpartum groups, with nonoverlapping 95% CIs of modeled trajectories between days 6 and 66 after surgery. CIs were more precise at most time periods with growth curve analysis compared with ANOVA.Conclusions: The authors describe a method of analysis to quantify recovery from hypersensitivity after surgery in rats with several distinct advantages over traditionally used methods. Study results do not support a sex difference in trajectory of recovery but confirm and extend previous observations that injury at the time of obstetric delivery is associated with an abnormally rapid recovery.