Identification of an operon required for ferrichrome iron utilization in Vibrio cholerae
Identification of an operon required for ferrichrome iron utilization in Vibrio cholerae
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DOI：
10.1128/jb.182.8.2350-2353.2000
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发表时间：
2000-04-01
影响因子：
3.2
通讯作者：
Calderwood, SB
中科院分区：
文献类型：
--
作者：
Rogers, MB;Sexton, JA;Calderwood, SB
用 TnphoA 对霍乱弧菌进行诱变，然后筛选在低铁条件下激活的融合体，从而鉴定出七种独立的融合菌株，每种菌株都缺乏利用铁铬作为唯一铁源在平板生物测定中生长的能力，并且在编码与大肠杆菌 FhuA 或 FhuD 同源的产物的基因中插入了插入片段。基因融合体的表达不依赖于 IrgB，但受 Fur 调节。我们在这里报告了操纵子图谱和基于核苷酸序列预测的 FhuA 氨基酸序列。与大肠杆菌 fhu 操纵子的基因不同，霍乱弧菌铁红利用基因与编码 ATP 结合盒转运蛋白同源物的基因相邻且方向相反，但该基因如果被破坏，不会影响体外铁红的利用。
Mutagenesis of Vibrio cholerae with TnphoA, followed by screening for fusions that were activated under low-iron conditions, led to the identification of seven independent fusion strains, each of which was deficient in the ability to utilize ferrichrome as a sole iron source for growth in a plate bioassay and had an insertion in genes encoding products homologous to Escherichia coli FhuA or FhuD. Expression of the gene fusions was independent of IrgB but regulated by Fur. We report here a map of the operon and the predicted amino acid sequence of FhuA, based on the nucleotide sequence. Unlike those of the E. coli fhu operon, the V. cholerac ferrichrome utilization genes are located adjacent and opposite in orientation to a gene encoding an ATP-binding cassette transporter homolog, but this gene, if disrupted, does not affect the utilization of ferrichrome in vitro.